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if you start with 2 pieces of DNA, after 4 cycles of PCR how many would...
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How many copies will be present after 4 replication cycles? a. 7 b. 64 c. 48 d. 24 e. 8
If you start with 47 DNA segments, how many total copies of DNA will you have after 12 cycles of PCR? Also what does attenuated mean in regards to the K12 strain of E. coli being used in lab? Is the answer “we have removed the gene that allows the bacteria to replicate”? Thank you!!
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
Assuming that you start with one template DNA molecule, how many total DNA molecules would you have after 30 cycles? O 6.48 x 105 060 0 1,073 O ~1.07 x 109 Submit Answer Tries 0/99
1. What are the degenerate primers in the PCR amplification protocol? What do they do? 2. Suppose you begin a PCR reaction with 1 piece of double stranded DNA. After 30 cycles of replication, how many pieces of double stranded DNA do you now have? 3. What would be the consequence of having too much DNA in the sample? Would it interfere with the PCR reaction? Why? 4. What happens during the annealing step of the PCR reaction? During the...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
PCR Question: after 25 cycles in the thermometer, the DNA template is amplified to _____ copies. Choices a) 25 to the power of 2 b) over 100,000 c) over a million d) over 33 million
Today we amplified 50 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 15,000 molecules of the insulin gene [50 ng DNA= 2.5 x 10-20mol; (2.5x10-20)x(6.023×1023) = 1.5 x 104molecules]. We performed PCR for 35 cycles to amplify the amount of this gene. a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2Nformula)? b. How many molecules of the insulin gene would, therefore, be present after PCR?
1) If a restriction enzyme cuts a circular DNA into five fragments, how many restriction sites are there in the DNA? 2) How many molecules of DNA will be present after 6 cycles of PCR, if you started with one double-stranded DNA molecule? CELL BIOLOGY QUESTIONS!! SHOW WORK PLEASE
5) p points) Temperature regulation is critical while preforming PCR. What could resuit IT the PCR reaction is not cooled to 42°C -65°C before the extension step? Why would this occur (2 points) If you start with one copy of a single gene of interest and perform PCR on that sequence, how many copies of the gene would you have after 10 cycles? Explain your reasoning.