Assuming that cells grow at a constant rate, if you originally placed 5x10^5 cells in a...
Assuming that cells grow at a constant rate, if you originally placed 5x10^5 cells in a flask and they double every 38 hours, how many cells would you expect to be in the flask after 5 days in culture?
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Assuming that cells grow at a constant rate, if you originally
placed 5x10^5 cells in a...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
BONUS (2pt): assuming that cells grow aerobically in a rich growth medium (all precursors are pro...
can you please help me with this question
BONUS (2pt): assuming that cells grow aerobically in a rich growth medium (all precursors are provided), how many molecules of glucose should be consumed to produce 1 molecule of 100 AA protein?
BONUS (2pt): assuming that cells grow aerobically in a rich growth medium (all precursors are provided), how many molecules of glucose should be consumed to produce 1 molecule of 100 AA protein?
MICROBIO HELP: You want to grow up some bacteria to bring to class to show your friends, and you ...
MICROBIO HELP: You want to grow up some bacteria to bring to class to show your friends, and you want to have enough to give everyone in the class 100 bacteria. If you are starting with only 5, how many generation times should you allow so you will have enough? (assume there are 130people in the class). If you knew it took 1 hour and 39 minutes to grow all your bacteria, what is the generation time? Helpful information provided:...
You are working with a population of multi-potent stem cells that you grow in a tissue...
You are working with a population of multi-potent stem cells that you grow in a tissue culture dish. They have the capacity to differentiate into any one of three possible differentiated cell types (alpha, beta, delta) if they are first exposed to a signaling factor (Bmp4) for 24 hours and then cultured without this factor for another 24 hours. The specific choice the cells make after treatment (alpha or beta or delta) is more or less random among the cells...
You are working with a population of multi-potent stem cells that you grow in a tissue...
You are working with a population of multi-potent stem cells that you grow in a tissue culture dish. They have the capacity to differentiate into any one of three possible differentiated cell types (alpha, beta, delta) if they are first exposed to a signaling factor (Bmp4) for 24 hours and then cultured without this factor for another 24 hours. The specific choice the cells make after treatment (alpha or beta or delta) is more or less random among the cells...
You are working with a population of multi-potent stem cells that you grow in a tissue...
You are working with a population of multi-potent stem cells that you grow in a tissue culture dish. They have the capacity to differentiate into any one of three possible differentiated cell types (alpha, beta, delta) if they are first exposed to a signaling factor (Bmp4) for 24 hours and then cultured without this factor for another 24 hours. The specific choice the cells make after treatment (alpha or beta or delta) is more or less random among the cells...
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106...
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106 cells. The cell yield is the total amount of adherent cells at confluence. How many adherent cells would you expect to be in this flask at 80% confluence? 2) For adherent cells, the seeding density is usually between what range of % confluencies? 3) How many total cells would you place in a T75 flask with a cell yield of 8.4 x 106 cells...
5. You grow a culture of E. coli to an OD reading of 0.8. Your instructor...
5. You grow a culture of E. coli to an OD reading of 0.8. Your instructor wants 40 colonies on a plate. Ideally, you want to only spread 100ul on a LB plate. How would you carry out the proper dilutions in order to arrive at 40 cells on a plate? Please show your calculations and all work. (1 O.D. = 1 x 108 cells/mL).
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...
You are given a flask with liquid media containing a population of S. pombe cells that...
You are given a flask with liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes...
can you please help me with this question
BONUS (2pt): assuming that cells grow aerobically in a rich growth medium (all precursors are provided), how many molecules of glucose should be consumed to produce 1 molecule of 100 AA protein?
BONUS (2pt): assuming that cells grow aerobically in a rich growth medium (all precursors are provided), how many molecules of glucose should be consumed to produce 1 molecule of 100 AA protein?
You are given a flask with liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes...
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