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When an ORF does not show up in a mutant library, what are two possible reasons?

When an ORF does not show up in a mutant library, what are two possible reasons?

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Answer #1

ORF does not show up in a mutant library because of following reasons:

* When there is mutation occur at third base of codon in the mRNA. This third base is wooble base pair. This wooble base can be recognise by same tRNA. Then there is no mutation in the library.

For example: third base of codon A, U Or C is recognised by first base of anticodon of tRNA and this tRNA transfer same amino acid.

*When mutation occurs in degenerative codon of mRNA. It tells that more than two codon codes for same amino acid.

For example : UUU and UUC encodes phenylalanine

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