What is another plating-based method for enumeration of a bacterial sample?
Three method to do enumerate the microorganisms are
1. Direct microscopic count
2. Standard plate count
3. Spread plate method
Pour Plate Procedure - Enumeration of Bacterial Cells in a Mixed
Sample
The number of microorganisms in a mixed sample which is added to a
molten agar medium. The process results in colonies uniformly
distributed throughout the solid medium when the appropriate sample
dilution is plated.
Spread plate method - Aliquots of diluted bacteria are spread onto surface of appropriate medium in Petri dishes with a sterilized glass rod.
What is another plating-based method for enumeration of a bacterial sample?
What is the importance of defining detection limits for different plating methods and enumeration of bacteria in particular?
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml in the original sample? What would it be if you plated 0.1 ml with same colonies formed?
C# Introductory programming question: What is an enumeration and what are the advantages of creating an enumeration type? Show an example of an enumeration definition.
Which bacterial quantitation method would you use for your pathogenic sample? Provide an explanation of why you chose that technique
Question 42 1 pts What carries a gene from one bacterial cell to another bacterial cell? A electrophoresis gel A restriction enzyme Polymerase chain reaction A plasmid Question 43 1 pts What is a genetically modified organism (GMO)? Any agricultural organism produced by breeding or biotechnology A hybrid organism A plant with certain genes removed An organism with an artificially altered genome
A bacterial sample has a concentration of 2 x106 CFUlml. Show a scheme of dilutions to obtain 30-300 colonies on a plate. Your scheme should contain the volume of diluent (sterile water), volume of sample transferred each time, the concentration of bacteria (CFU/mL) in each dilution. You can assume you are plating 1 mL of the dilution on plates of nutrient agar. See Figure 1 in protocol 10. Dilution factor in single transfer (change x and y to numbers 10...
What role does a bacteriophage play in transferring DNA from one bacterial cell to another?
Tody very important , Thanks Solve the following assignment Problem by complete enumeration method. Jobs/workers B с 1 100 60 90 2 80 70 120 3 60 90 100 3
I have to do a serial dilution for plating bacteria, and I am given a sample that has been diluted to 10-4 already. I will need to plate the 10-5 and 10-7 dilutions. What will be the steps I will have to do? Note: I cannot use any more tubes/saline outside of what I have been given. Materials: 2 tubes labelled 10-5 and 10-7. 20ml of sterile saline 5 ml of the 10-4 diluted sample. Sterile pipettes
How do pUC - type plasmids confer ampicillin - resistance to their host bacterial cells? 5) What is the purpose of plating host cells on ampicillin - containing plates?