How do pUC - type plasmids confer ampicillin - resistance to their host bacterial cells? 5)...
How do pUC - type plasmids confer ampicillin - resistance to their host bacterial cells?
5) What is the purpose of plating host cells on ampicillin - containing plates?
Homework Answers
Ans 4:- pUC containing ampicillin resistance, when such plasmid is introduced into the bacterial cells they show resistance to an amphicilin drug, the resistance is due the activity of β-lactamase toxicity of the drug is reducing and hence bacteria are allowed to grow in such conditions.
Ans 5:- plating host cells on ampicillin-containing plates act as a selectable marker thus select only those bacteria which are transformed this is because when antibiotics are added to the medium only those bacteria will live which have the resistance gene while as those bacteria lacking this gene donot survive.
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How do pUC - type plasmids confer ampicillin - resistance to
their host bacterial cells?
5)...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
How can ampicilin resistance gene and the hygromycin resistance gene help us to select for bacterial...
How can ampicilin resistance gene and the hygromycin
resistance gene help us to select for bacterial cells containing
the pOTC- delta plasmid?
CHICOU COUPLUNDU Nhel Kpn 1 1569 1601 Not 1 KpnI 1030 988 2295/ 2310 ОТСА - Sphl 2559 Nde1 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405-6265 Hygro: Hygromycin resistance gene 12933 - 3943
Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help...
Q1) How can these selection markers ( Ampicillin resistance gene and the Hygromycin resistance gene ) help us to select for bacterial cells containing the pOTC-Δ plasmid? (1 mark )
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid...
E. coli cells that are sensitive to ampicillin are mixed with many copies of a plasmid that has a gene for ampicillin resistance and then plated on medium containing ampicillin. What will happen next? 1.Only E. coli cells that have not taken up a plasmid will grow and form colonies. 2.only E. coli cells that have taken up a plasmid will grow and form colonies. 3.E. coli cells with and without a plasmid will grow and form colonies. 4.The plasmid...
Please answer these four questions. 6C. A type of plasmid used frequently as a vector in...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
d. Ampicillin resistance gene and the hygromycin resistance gene e. Ndel and Kpnl f pUC Ori and OTC-A o How can these selection markers help us to select for bacterial cells containing the pOTCA plasmid? (1 mark) ered
How can ampicilin resistance gene and the hygromycin
resistance gene help us to select for bacterial cells containing
the pOTC- delta plasmid?
CHICOU COUPLUNDU Nhel Kpn 1 1569 1601 Not 1 KpnI 1030 988 2295/ 2310 ОТСА - Sphl 2559 Nde1 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405-6265 Hygro: Hygromycin resistance gene 12933 - 3943
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
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