Question

>AEH84521.1 protein of unknown function DUF150 [Mesorhizobium opportunistum WSM2075]

MTAMTSEGDDRIIRESGIDARIALIIQPVLRGIGFRLVRVHLSGQNGLTLQIMAEREDGTMTVEDCEEVS

RAVSPALDVDDPIEKAYHLEVSSPGIDRPLVRKSDFVTWTGHLVKMETSVVVADRKRFKGKIAEAGENDV

LIERDKAAYGEEPTVRVPYDAISETRLILTDDLIRDALSKDNRARKEAKKRRGEPDDDVPEGTEADATEE

HEQES

What is the dominant biochemical activity

or

function

that your protein is predicted to

do

. Which sites on the protein contribute

to the process directly? Which sites contribute indirectly? How does the

predicted

function

relate to

the

biological function

of the cell

? What is expected to occur to

the organism if the gene that generates the

protein is inactivated?

Describe the

predicted structure of the protein.

Give a protein structure model of your protein.

If

applicable,

name the

operon

the gene of your protein belongs to

?

Answer 1

This protein based on the blast result is " ribosome maturation factor". This protein is required for the maturation of 30S subunit of ribosomes in prokaryotes. This maturation of ribosomes help in the ribosomes activation , so that protein translation could carry out. The sites present on the protein that help in processing their function is the amino acid between 103- 133 amino acid, that is required for the 30S subunit activation. The other sites is the amino acid between 3- 50 amino acid

Result of NCBI blast

If the gene required for the expression of this protein is inactivated , then the ribosome 30S subunit will not able to bind with mRNA and hence protein translation could not take place.

The 3D structure of the protein is