Nuen is a protein only found in neurons. What is one promoter that is on only in neurons? What would be a good marker for neurons?
Answer: NeuN (Neuronal Nuclei): It is a neuronal nuclear antigen of approx 46 to 48 kDa weight which is exclusively present in neurons. It is also utilised as a marker of neurons. It is similar to the protein Fox-3/Rbfox3 (a mammalian homologue as Fox-1). Tac-1 and Dlx5/6 (CREB-binding protein) are the promoters only present in neurons. Neurol subtypes express a specific type of transcription factor, enzymes, receptors and cytoskeleton protein. The selection of markers depends on types of study like for immature neuron NeuroD1, TBR1 and Stathmin-1 are the markers, for neural epithelial- SOX2, HES1, HES-3 and E-cadherin are the markers, for mature neurons NeuN, MAP2 Synaptophysin etc. Are the specific markers
Nuen is a protein only found in neurons. What is one promoter that is on only...
A cell is found to have a high degree of methylation on the promoter for the Rb gene. True or False: This methylation pattern will impact protein primary sequence. True False A cell is found to have a high degree of methylation on the promoter for the Rb gene. Would this methylation pattern impact gene expression? (select one best answer) Transcription would increase Translation would increase No change in expression would occur Transcription would decrease
Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene is diagrammed to the right.? 2) Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene...
The nicotinic acetylcholine receptors are transmembrane protein found in the plasma membrane of human neurons. You are studying a member of the receptor family that is glycosylated. Based on this information, answer the following questions: Was the receptor synthesized on cytosolic ribosomes or ER-bound ribosomes? Justify your answer Describe the cellular path that the receptor likely took from synthesis to the plasma membrane.
Question 17 3 pts Neurons cells are found in the nervous system and provide direct protection and physical support to the neurons. One protein made by these cells is myelin that is essential in neurological function as seen in epilepsy where the degeneration of the protein is observed and patients experience neurological symptoms. J Oection 10 Ante Question 15 2 pts Which one of the following establishes and maintains the resting potential of cells across the cell membrane? Na+ leak...
Protein in the brain (neurons) specifically and one in the muscles tissue where encoded by same gene. How did that happen ? 1-alternative mRNA splicing 2-tissue-specific splicing factor 3-both 1&2
Only neurons that touch one another can communicate
-Which elements are found in a eukaryotic promoter vs. a prokaryotic promoter? -what is the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis -what are the steps and process of thermal cycling? -what is the restriction enzyme(s) and how do you know when they leave blunt or sticky ends (ie. XbaI, SmaI, EcoRI, BamHI)? -The lac and trp operons (form a figure showing the operon). - What is RNA silencing involvee (in general, what is RNA interference;...
3. You make an empty lipid vesicle whose sole membrane protein is a version of the sodium-potassium pump found in neurons, that has been modified to pump one Na ion every time it pumps one K ion. It is oriented in the membrane the same way it is in neurons a) What would you expect to happen if you placed this vesicle in a solution containing only an equal concentration of Na* and K* ions, and filled the vesicle with...
A mutation occurs that causes the promoter of a gene to over-express a protein. Would this most likely be a gain of function or loss of function mutation? Why?
The native conformation of a protein: 1- is found only when the protein is first formed, but not afterwards. 2- none of the other answers are true. 3- has biological activity 4- is any possible conformation of the protein