ANS) qPCR that is quantitative polymerase chain reaction is a sensitive technique that helps in determining protein to protein interaction because they work on real time basis. The qPCR illuminates each protein molecules with a different wavelength to study their interactions.
Can you determine protein-protein interation from qPCR? If so, please explain how.
Assume that you are using qPCR to determine the amount of DNA in a given sample. Sample 1, from which you know that it contained an amount of 1 pg, required 15 PCR cycles to reach a specific level of PCR product. Your sample 2 required only 5 PCR cycles to reach the same level. Sample 3 required 18 PCR cycles to come to the same level of PCR product. How much DNA did samples 2 and 3 contain? RT-qPCR...
explain how you can guess if a specific protein is a transmembrane protein.
Please explain in detail how to determine the answer : If a part of a protein has sequence of GLEDKSA and forms one continuous α-helix, which amino acid is the carbonyl of glycine non-covalently boned to?
The answer is C, can someone please explain why this is the answer? You purify bee (Apis mellifera). After a year of research, you determine that the enzyme is essential for a pathway that allows the organism to produce venom. You are now interested in how the enzyme is regulated, so you conduct a series of experiments to help you address this aim. You finally determine that the enzyme is inhibited under conditions that prevent its dephosphorylation. What do these...
Please explain how to do this so I can complete the other questions - Thank you! Express your answer as an integer. PO ALQ O a ? 3.42 pm Submit Request Answer
Please explain and show all steps so I can study from it. You measure the elastic modulus of a stack of pancakes and determine it to be 20000 Pa. If you assume the interaction energy between the glutenin molecules is Uint=100kBT=4×10-19J, what is the characteristic bond length? Please report your answer in nanometers (nm). Recall there are 1,000,000,000 nm in one meter. to solve this problem, you need to use the Equation of the Week: E= Uint ------ l3
Problem 10 (3 points): Assume that you are using qPCR to determine the amount of DNA in a given sample. Your calibration sample, which contained an amount of 10-15 mol, required 12 PCR cycles to reach a specific level of PCR product. Your cell sample with an unknown DNA amount required only 9 PCR cycles to reach the same level. How many moles of DNA did the cell sample contain? Describe how you arrived at that conclusion.
Explain how Niacin can be used to reduce a ketone. Please provide a mechanism. Draw by hand Explain how a cell ubiquitinates a protein and provide two possible reasonings for doing so. Explain how high levels of amino acids can act as feedback inhibitors.
Explain the concept of an organelle marker protein or enzyme. How can a marker enzyme/protein aid in isolating and enriching a preparation for an organelle? A student prepares mitochondria from a cauliflower which has been stored at 4°C for three weeks and finds the SDH specific activity of their PMS is about the same as their mitochondrial fraction. The same protocol used on fresh cauliflowers showed a 10-fold higher specific activity in the mitochondrial fraction compared to the PMS. Can...
please explain answer so i can understand how to slove for further problems! thank you !! 1. A compound has a molecular formula of CaH:02. Propose a valid structure that matches well with the IR spectrum below. Please identify the main functional group(s) on the spectrum below. RIVENURSER identify the main functional group(s) on the spectrum below. RAVENUMBERI-