explain how you can guess if a specific protein is a transmembrane protein.
Transmembrane proteins are the proteins that are present in the plasma membrane. The plasma membrane is an amphipathic structure. It has a hydrophilic head group and hydrophobic tail group. Any protein that is present in the plasma membrane has to pass through the hydrophobic tail.
Based on the polarity amino acid are divided into polar and non-polar amino acid. The non-polar amino acid is hydrophobic in nature and interacts with each other by hydrophobic interactions. Tail group of the plasma membrane are also hydrophobic. So hydrophilic amino acid can make the transmembrane domain.
If we want to guess if the specific protein has a transmembrane domain then we need to look for a stretch of hydrophobic amino acid. If the stretch is present then we can guess that protein is a transmembrane protein.
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explain how you can guess if a specific protein is a transmembrane protein.
How would you describe the polarity of the enzyme glucose-6-phosphatase, knowing it is a transmembrane protein?
6a. You have found a new protein of interest: this protein has a transmembrane domain (as predicted by hydropathy analysis). You want to confirm this and determine which portion of the protein is outside the cell and which portion is inside the cell. You begin with a protease protection assay, the results are below. Does this protein most likely have a transmembrane domain? Is the larger portion inside the cell or outside the cell? Is there anything wrong with the...
Please also explain why! Thank you!
18) You want to design an ER transmembrane protein with three transmembrane domains with the N-terminal in the ER lumen and the C-terminal in the cytosol. Which of the following arrangement(s) of signals will be required to attain such a topology? A. From N-terminal: signal sequence, start-transfer signal, stop-transfer signal. B. From N-terminal: signal sequence, start-transfer signal, stop-transfer signal, start-transfer signal. C. From N-terminal: signal sequence, stop-transfer signal, start-transfer signal. D. From N-terminal: start-transfer...
1. Transmembrane proteins are embedded in cell membranes. If one transmembrane protein is pulled away from the cell membrane using a magnetic force microscope, and the force required to extract this protein is: fIpN)-15(x/nm)+(x nm) where r is the distance between the protein and membrane. The protein is pulled from the fully embedded state (x 0) to fully unbound state (x2-5 nm) 1.1 What is the work needed to extract this transmembrane protein? 1.2 What would be the work (in...
1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell? A. Extract all the proteins from the cell and analyze them on a protein gel. B.Use Polymerase Chain Reaction (PCR) to amplify the protein. C. Use an fluorescent antibody to the protein and allow it to bind to the cells. D Isolate the RNA for the protein and use...
Explain the concept of an organelle marker protein or enzyme. How can a marker enzyme/protein aid in isolating and enriching a preparation for an organelle? A student prepares mitochondria from a cauliflower which has been stored at 4°C for three weeks and finds the SDH specific activity of their PMS is about the same as their mitochondrial fraction. The same protocol used on fresh cauliflowers showed a 10-fold higher specific activity in the mitochondrial fraction compared to the PMS. Can...
Can you determine protein-protein interation from qPCR? If so, please explain how.
A protein has 8 stop transfer signals. How many transmembrane domains does it have? A. 1 B. 4 C. 8 D. 16
Explain how a tissue-specific RNA-binding protein can lead to tissue-specific alternative splicing via splicing enhancers or splicing silencers. A.Trans-acting sequences that promote (splicing enhancers) or inhibit (splicing silencers) splicing are recognized by different classes of RNA-binding proteins (RBPs). B. Trans-acting sequences that promote (splicing silencers) or inhibit (splicing enhancers) splicing are recognized by the same class of RNA-binding proteins (RBPs) in different tissues. C. Cis-acting sequences that promote (splicing enhancers) or inhibit (splicing silencers) splicing are recognized by different classes...
3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when introduced to the plasma membrane after trafficking?