Question

1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell?

A. Extract all the proteins from the cell and analyze them on a protein gel.

B.Use Polymerase Chain Reaction (PCR) to amplify the protein.

C. Use an fluorescent antibody to the protein and allow it to bind to the cells.

D Isolate the RNA for the protein and use it to bind back to the cell.

2. What would most likely happen if the Na+K+ Pump (ATPase) stopped functioning in the cell?

A. The cell would swell by taking up water.

B. The cell would shrink by pumping out water.

C. The inside of the cell would be more negative.

D. Nothing would happen to the cell.

3. The sodium potassium pump uses ATP for energy to make the transport thermodynamically favorable. What is happening to the ATP during transport?

A. The ATP binds to the ion binding sites along with the ions, initiating transport.

B. The pump is phosphorylated and its conformation is changed.

C. PPi (pyrophosphate or two phosphates) are release from the ATP the the pump changes confirmation.

D. The ATP binds to the transmembrane domain of the transporter and changes it confirmation.

Answer 1

1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell?

->Proteins are being tracked in living cells by using a fluorescent marker called green fluorescent protein (GFP). Tagging proteins with GFP is as simple as attaching the gene for GFP to one end of the gene that encodes a protein of interest.

So, Use an fluorescent antibody to the protein and allow it to bind to the cells.

2. What would most likely happen if the Na+K+ Pump (ATPase) stopped functioning in the cell?

->Every cycle of an Na/K pump removes three cations (3 Na+) for every two (2 K+) that it imports into the cell. Thus there is a net loss of one cation for every cycle of the pump. ... Therefore, without these pumps, the cell swells up.

So the cell would swell by taking up water.

3. The sodium potassium pump uses ATP for energy to make the transport thermodynamically favorable. What is happening to the ATP during transport?

->Inorganic pyrophosphatase (PPase) cleaves the pyrophosphate (PPi) released during the priming reaction into phosphate (Pi).

So , PPi (pyrophosphate or two phosphates) are release from the ATP the the pump changes confirmation.