You have purified a new protein from the blood serum of a cancer patient. Propose an...
Homework Answers
1. Digest the protein using proteases such that overlapping fragments are obtained, trypsin can be used.
2. Peptide fragments obtained can be used for MS MS mass spectrometer to determine the relative mass of each amino acid.
3. Edmann degradation can also be used to determine the Amino acid sequence of the protein.
4. Once sequence is obtained, using bioinformatics we can determine its pI and about which class of protein it belong depending on the homology.
Add Answer to:
You
have purified a new protein from the blood serum of a cancer
patient. Propose an...
In this experiment, you will carry out ALP analyses of two simulated blood serum samples: a...
In this experiment, you will carry out ALP analyses of two simulated blood serum samples: a "normal" (in terms of ALP level) serum sample, and a hypothetical patient serum sample. You will determine the amount of ALP in each sample, using kinetic enzyme assays and graphing techniques. You will then draw conclusions about possible bone or liver disease in the hypothetical patient. Normal Time Patient Time Normal Abs Abs 0.00 0.07 0.10 y=0.0232 +0.0032 0.16 0.00 0.03 0.04 0.06 0.09...
You are studying a particular kind of Ca2+ transport protein in a purified system (the protein...
You are studying a particular kind of Ca2+ transport protein in a purified system (the protein is the only transporter in the membrane). You find that if you add ATP to the interior of the membrane system, there is a net flux of Ca2+ out into the buffer (i.e., out of the 'cell'). Based on this result, what kind of transporter do you think you are studying? Electrogenic lon channel Active Transporter Coupled Active Transporter • Facilitated Diffusion Uniporter
Question 1. You are a cancer researcher studying skin cancer cells that have become metastatic (ie....
Question 1. You are a cancer researcher studying skin cancer cells that have become metastatic (ie. they've migrated from the site of the original tumor to establish new secondary tumors). You analyze skin samples from an unaffected patient and from one with the metastatic cancer. After a series of detergent solubilization and centrifugation steps, you separate pellet samples from each tissue type by gel electrophoresis and find the following results: skin normal cancer tissue tissue 250 Da 120kDa 75kDa- 60kDa...
Question 1. You are a cancer researcher studying skin cancer cells that have become metastatic (i.e....
Question 1.
You are a cancer researcher studying skin cancer cells that have
become metastatic (i.e. they’ve migrated from the site of the
original tumor to establish new secondary tumors). You analyze skin
samples from an unaffected patient and from one with the metastatic
cancer. After a series of detergent solublization and
centrifugation steps, you separate pellet samples from each tissue
type by gel electrophoresis and find the following results:
A. What is the difference between the normal skin sample...
Two proteins have been purified by a researcher, called Protein A and Protein B. She determines...
Two proteins have been purified by a researcher, called Protein A and Protein B. She determines the extinction coefficient for each, generating the following information: Protein A: 1 mg/ml solution OD280=1.1 Protein B: 1 mg/ml solution OD280=0.27 She then purifies a new preparation of Protein A. Her new preparation of Protein A has 4 ml total, and when it is diluted 1/10 it gives OD280=0.44. What is the total protein in her new preparation of Protein A in mg?
9. Rational Treatment of Cancer (16 pts total) You are a cutting edge oncologist who has treated a cancer patient with I...
9. Rational Treatment of Cancer (16 pts total) You are a cutting edge oncologist who has treated a cancer patient with Ireesa. The patient went into remission for several years but their cancer has now come back. You also have a cancer research lab in which you study drugs like Ireesa. You are also in the process of applying to the FDA for approval for a new clinical trial but you must first answer the following questions: a) What type...
PLEASE HELP THANK YOU!!! A new protein of an unknown structure has been purified. gel filtration...
PLEASE HELP THANK YOU!!! A new protein of an unknown structure has been purified. gel filtration revealed that the native protein had a molecular weight of 240,000 Da whereas SDS-PAGE electrophoreses shows two peaks of 80,000 da and 40,000 da. The results of the same experiments performed in presence of dithiothreitol are identical to results obtained in absence of DTT. explain what can be determine about the structure of this protein.
You have purified a His-tagged protein expressed in human cell culture by Ni2+ affinity chromatography. You...
You have purified a His-tagged protein expressed in human cell
culture by Ni2+ affinity
chromatography.
You have purified a His-tagged protein expressed in human cell culture by Ni^2+ affinity chromatography. When you run the sample on an SDS PAGE gel in the absence of reductant you observe a single band; in the presence of reductant, however, you observe two bands. If you treat with reductant and pour the products over the nickel column, you detect one band in the flow-through...
You have purified a sample of your protein of interest and have measured the A280 of a 1 in 3 dilution of the protein sample (1 cm pathlength). The value you have obtained for A280 was 0.576. Given th...
You have purified a sample of your protein of interest and have measured the A280 of a 1 in 3 dilution of the protein sample (1 cm pathlength). The value you have obtained for A280 was 0.576. Given that you do not know the sequence of your protein, calculate the approximate concentration of your original protein sample in mg/mL. Give your answer to 2 s.f.
Above you have three patients with liver cancer. In the top row (3 boxes), band on...
Above you have three patients with liver cancer. In the top row (3 boxes), band on the left is the control (Showing a protein that is being over expressed. In the top row (3 boxes), band on the right is the expression of the patient with liver cancer. Which patient/patients do you see expression of the myc receptor (oncogene that causes liver cancer). Are any of the patients cancer free? If not explain, if so explain. The bottom row (3...
In this experiment, you will carry out ALP analyses of two simulated blood serum samples: a "normal" (in terms of ALP level) serum sample, and a hypothetical patient serum sample. You will determine the amount of ALP in each sample, using kinetic enzyme assays and graphing techniques. You will then draw conclusions about possible bone or liver disease in the hypothetical patient. Normal Time Patient Time Normal Abs Abs 0.00 0.07 0.10 y=0.0232 +0.0032 0.16 0.00 0.03 0.04 0.06 0.09...
You are studying a particular kind of Ca2+ transport protein in a purified system (the protein is the only transporter in the membrane). You find that if you add ATP to the interior of the membrane system, there is a net flux of Ca2+ out into the buffer (i.e., out of the 'cell'). Based on this result, what kind of transporter do you think you are studying? Electrogenic lon channel Active Transporter Coupled Active Transporter • Facilitated Diffusion Uniporter
Question 1. You are a cancer researcher studying skin cancer cells that have become metastatic (ie. they've migrated from the site of the original tumor to establish new secondary tumors). You analyze skin samples from an unaffected patient and from one with the metastatic cancer. After a series of detergent solubilization and centrifugation steps, you separate pellet samples from each tissue type by gel electrophoresis and find the following results: skin normal cancer tissue tissue 250 Da 120kDa 75kDa- 60kDa...
Question 1.
You are a cancer researcher studying skin cancer cells that have
become metastatic (i.e. they’ve migrated from the site of the
original tumor to establish new secondary tumors). You analyze skin
samples from an unaffected patient and from one with the metastatic
cancer. After a series of detergent solublization and
centrifugation steps, you separate pellet samples from each tissue
type by gel electrophoresis and find the following results:
A. What is the difference between the normal skin sample...
You have purified a His-tagged protein expressed in human cell
culture by Ni2+ affinity
chromatography.
You have purified a His-tagged protein expressed in human cell culture by Ni^2+ affinity chromatography. When you run the sample on an SDS PAGE gel in the absence of reductant you observe a single band; in the presence of reductant, however, you observe two bands. If you treat with reductant and pour the products over the nickel column, you detect one band in the flow-through...
Above you have three patients with liver cancer. In the top row (3 boxes), band on the left is the control (Showing a protein that is being over expressed. In the top row (3 boxes), band on the right is the expression of the patient with liver cancer. Which patient/patients do you see expression of the myc receptor (oncogene that causes liver cancer). Are any of the patients cancer free? If not explain, if so explain. The bottom row (3...
Most questions answered within 3 hours.
-
An explosion breaks a 20.0-kg object into three parts. The
object is initially moving at a...
asked 9 minutes ago -
Calculate the approximate number of residues of Rubisco, which
is involved in carbon fixation in plants,...
asked 1 hour ago -
Other decisions about scientific claims can have a much broader
impact.ENERGYarrow-10x10.png, environment, health, security - all...
asked 2 hours ago -
I need to write a research paper and work cited about this
topic: The United States...
asked 2 hours ago -
Hello! I was wondering if I could have some help?
If the vapor pressure of carvone...
asked 2 hours ago -
An economist wants to estimate the mean per capita income (in
thousands of dollars) for a...
asked 3 hours ago -
What would be the input/output characteristic of a circuit
obtained by putting two of your 2's-complementers...
asked 3 hours ago -
In Drosophila, the transition from the syncytial blastoderm
stage to the cellular blastoderm stage is a...
asked 3 hours ago -
Project management question:
Name 3 different types of resources (hint: humans are one
type)
asked 3 hours ago -
Consider the following reaction: C 2H 2( g) + 2H 2( g) C 2H 6(
g)...
asked 3 hours ago -
Consider a 1.0 L buffer containing 0.092 mol L-1 HCOOH and 0.100
mol L-1 HCOO-. What...
asked 4 hours ago -
Koch Realty has owned a vacant land with a FMV of
$775,000 and an adjusted basis...
asked 4 hours ago