Homework Answers
Over expression of myc leads to cancer i.e. in the left side of top boxes. It is a transcription factor, which causes the cell proliferation and leads to cancer. Patient two i.e. second box having more expression of myc receptor so it has the more cell proliferation. Patient sample in box 3 is normal he has very low level of expression.
Actin used as a internal controle to see the equelloading into gel during SDS PAGE running.
By this we can assume that the protein loaded into each well is in equel amount. So that the variation in expression is countable compared to Actin also with control.
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Above you have three patients with liver cancer. In the top row (3 boxes), band on...
Please explain what might causes the following results A. You have bright band on a Northern...
Please explain what might causes the following results A. You have bright band on a Northern Blot for a specific RNA, but the Wester blot Shows no band for the protein encoded by that RNA. B. Your Western blot show that your protein increases size in extracts from cancer cell vs. normal cells. C. Your Northern blow shows different sized fragments in tissue extracted from brain cells vs heart muscles.
)You have labelled RNA probes ("riboprobes") directed against the sixth exon of a 7 exon long...
)You have labelled RNA probes ("riboprobes") directed against the sixth exon of a 7 exon long mRNA corresponding to the Nealosin gene. On a northern blot, you get the appropriate-sized band of 1235 bases from control liver tissue reliably Another scientist in your lab has recently obtained liver tissue from a cancer patient. Since he has some RNA from this sample left over, you decide to throw it on a gel with your sample and your Neolosin-specific riboprobe. In the...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify a novel protein from mammalian cells. You do not know this protein's size or charge, and are thus unsure of which steps to take. Fortunately, an antibody for this protein is available. You have two cell lines: one that expresses the protein, and one • that does not. . ) 4 5 III111 You run both cell lines on an SDS-PAGE (A) followed by...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is constitutively active in patients with Chronic Myelogenous...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is
constitutively active in patients with Chronic Myelogenous Leukemia
(CML). The structure of Gleevec is shown below:
a) Based on our discussion in lecture and the structure above,
explain how the “lead” compound structure for Gleevec was designed
and how it led to the development a compound that could bind ABL
with higher affinity than ATP.
b) Using a broad kinase inhibition assay, you discover that
Gleevec inhibits another tyrosine kinase called...
Pls give short answers. Thank you Q5 EGF pathway 23 Points A EGF EGFR GRB2 SOS...
Pls give short answers. Thank you
Q5 EGF pathway 23 Points A EGF EGFR GRB2 SOS KRAS Active RAS KRAS GOP GDP GTP RAF РІЗК MEK АКТ) ERK Growth and Suppression Proliferation of Apoptosis Shown is the EGF signaling pathway. This is a pathway that normally promotes growth during development, and inhibits apoptosis (or programmed cell death). This pathway is also involved in cancer. Epidermal growth factor (EGF) binds to its receptor (EGFR). This binding activates the protein KRAS, which...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I am having difficulty...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple terms! I am having difficulty understanding...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
)You have labelled RNA probes ("riboprobes") directed against the sixth exon of a 7 exon long mRNA corresponding to the Nealosin gene. On a northern blot, you get the appropriate-sized band of 1235 bases from control liver tissue reliably Another scientist in your lab has recently obtained liver tissue from a cancer patient. Since he has some RNA from this sample left over, you decide to throw it on a gel with your sample and your Neolosin-specific riboprobe. In the...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify a novel protein from mammalian cells. You do not know this protein's size or charge, and are thus unsure of which steps to take. Fortunately, an antibody for this protein is available. You have two cell lines: one that expresses the protein, and one • that does not. . ) 4 5 III111 You run both cell lines on an SDS-PAGE (A) followed by...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is
constitutively active in patients with Chronic Myelogenous Leukemia
(CML). The structure of Gleevec is shown below:
a) Based on our discussion in lecture and the structure above,
explain how the “lead” compound structure for Gleevec was designed
and how it led to the development a compound that could bind ABL
with higher affinity than ATP.
b) Using a broad kinase inhibition assay, you discover that
Gleevec inhibits another tyrosine kinase called...
Pls give short answers. Thank you
Q5 EGF pathway 23 Points A EGF EGFR GRB2 SOS KRAS Active RAS KRAS GOP GDP GTP RAF РІЗК MEK АКТ) ERK Growth and Suppression Proliferation of Apoptosis Shown is the EGF signaling pathway. This is a pathway that normally promotes growth during development, and inhibits apoptosis (or programmed cell death). This pathway is also involved in cancer. Epidermal growth factor (EGF) binds to its receptor (EGFR). This binding activates the protein KRAS, which...
90170021 201901/P53 pd 8. (3) Lator you find out that you have access to a microarray for 10 gones that are associated with John's type of cancer. The microarray was done comparing normal tissue the microarray are shown below. One of the patients is John sequence of proteins. What are these mutations? How do the mutations change the 9. (2) If you were to suggest gene therapy to the family. how you describe what the main goal of this therapy...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
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