Biochemistry Styrer Chapter 3 Problem 1. 1. Valuable reagents. The following reagents are often usedin protein...
Biochemistry Styrer Chapter 3 Problem 1.
1. Valuable reagents. The following reagents are often usedin protein chemistry: CNBr Trypsin Urea Performic acid Mercaptoethanol 6 N HCl Chymotrypsin Phenyl isothiocyanate Which one is the best suited for accomplishing each of thefollowing tasks?
(a) Determination of the amino acid sequence of a small peptide.
(b) Reversible denaturation of a protein devoid of disulfidebonds. Which additional reagent would you need if disulfide bonds were present?
(c) Hydrolysis of peptide bonds on the carboxyl side ofaromatic residues.
(d) Cleavage of peptide bonds on the carboxyl side of methionines .
(e) Hydrolysis of peptide bonds on the carboxyl side oflysine and arginine residues.
Homework Answers
(a) Determination of the amino acid sequence of a small peptide.= phenyl isothiocyanate
(b) Reversible denaturation of a protein devoid of disulfide bonds. Which additional reagent would you need if disulfide bonds were present = Mercaptoethanol, performic acid
(c) Hydrolysis of peptide bonds on the carboxyl side of aromatic residues.= chymotrypsin
(d) Cleavage of peptide bonds on the carboxyl side of methionines .= CNBr
(e) Hydrolysis of peptide bonds on the carboxyl side oflysine and arginine residues = Trypsin
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Biochemistry Styrer Chapter 3 Problem 1.
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