Question

The following DNA molecule is replicating and is going to undergo proofreading. To this end (in...

The following DNA molecule is replicating and is going to undergo proofreading. To this end (in bold), which enzymatic activity would do the proofreading?

P AGGCTAGACTCGTAGA OH

3' end AATCTGAGCAUCU

A. DNA polymerase I, 3' -exonuclease

B. DNA polymerase I, 5' -exonuclease

C. DNA polymerase III, 5' -exonuclease

D. DNA polymerase III, 3' -exonuclease

E. DNA repair system 3' to 5'

F. Two of the above

G. None of the above

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Answer #1

Answer will be two of the above which are both Pol I and iii 3'-⅝' exonuclease activity.

Because when DNA IS POLYMERIZED THEN THE END IS 3' .IF THE BASE IS INCORRECT THEN EXCISION IS STARTING FROM 3' END TO 5' END THAT WHY IT IS 3'-5'.

BUT IN REPLICATION POL III 3' WILL PROOFREAD THIS MISTAKE INSTANTLY.

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