True or False: DNA polymerase I performs proofreading by requiring a higher concentration of the next...
True or False: DNA polymerase I performs proofreading by requiring a higher concentration of the next dNTP following a mismatch allowing for unwinding of the nascent duplex and 3' --> 5' exonuclease digestion. If false explain why.
Homework Answers
True..DNA polymerase 1 performs proofreading by requiring a higher concentration of the next dNTP following a mismatch allowing for unwinding of the nascwnt duplex and 3'-5' exonuclease digestion.
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True or False: DNA polymerase I performs proofreading by
requiring a higher concentration of the next...
I. True or False, 25 points (5 each), Read the following statements and indicate whether the statement is true or false. For extra credit (5 points each) explain how the false statements are not...
I. True or False, 25 points (5 each), Read the following statements and indicate whether the statement is true or false. For extra credit (5 points each) explain how the false statements are not true. 3. DNA polymerase I performs proofreading by requiring a higher concentration of the next dNTP following a mismatch, allowing time for unwinding of the nascent duplex and 3 5' exonuclease digestion. 4. The DNA duplex of the genome is flexible enough to allow many values...
The following DNA molecule is replicating and is going to undergo proofreading. To this end (in...
The following DNA molecule is replicating and is going to undergo proofreading. To this end (in bold), which enzymatic activity would do the proofreading? P AGGCTAGACTCGTAGA OH 3' end AATCTGAGCAUCU A. DNA polymerase I, 3' -exonuclease B. DNA polymerase I, 5' -exonuclease C. DNA polymerase III, 5' -exonuclease D. DNA polymerase III, 3' -exonuclease E. DNA repair system 3' to 5' F. Two of the above G. None of the above
Can someone help explain complete the following sentences on E coli DNA polymerase In the DNA...
Can someone help explain complete the following sentences on E coli DNA polymerase In the DNA polymerase active site, a 3’ –O- nucleophile attacks a phosphate in the incoming dNTP to elongate the growing strand. Here, the reaction intermediate is stabilized by this metal ion: The metal ion described in (a) is stabilized at the DNA polymerase active site by binding to side chains of which amino acid: The 3-5’ exonuclease reaction is important for which process? Which DNA polymerase(s)...
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function...
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3' exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA...
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase ! High fidelity of DNA replication is ensured by 3-5 exonuclease activity of DNA polymerases Next
i need help in biochem Which of the following statements is true? Exonuclease III cleaves DNA...
i need help in biochem Which of the following statements is true? Exonuclease III cleaves DNA in a sequence specific manner Bacteriophage lambda exonuclease adds homopolymer tails to the 3’ –OH ends of a linear duplex Alkaline phosphatase removes terminal phosphates Kinase removes nucleotides from 5‘ends (5 points) Arrange the following steps involved in Gene cloning in the correct order a) Expression of recombinant gene in host b) Insertion of isolated gene to the vector c) Extraction of recombinant gene...
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic...
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic RNA usually undergoes nuclear processing C. Polypeptides are synthesized by addition of amino acids to the amino terminus. D. The 3' end of mRNA corresponds to the carboxyl terminus of the protein. Grade 2. Which of the following A. It may be autocatalytic. B. Spliceosomes are present in organelles and nuclei C. It involves removal of exons is true regarding RNA processing? D. It...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in polymerase cannot extend a new A) It is necessary for DNA synthesis because DNA polymerase canno strand in the 3 to 5' direction. B) It proofreads bases added by DNA polymerase. C) It is a type of RNA polymerase. D) It has terminal transferase activity. 28. How many of the following statements about PCNA are TRUE? • It encircles ssDNA. • It is an...
I. True or False, 25 points (5 each), Read the following statements and indicate whether the statement is true or false. For extra credit (5 points each) explain how the false statements are not true. 3. DNA polymerase I performs proofreading by requiring a higher concentration of the next dNTP following a mismatch, allowing time for unwinding of the nascent duplex and 3 5' exonuclease digestion. 4. The DNA duplex of the genome is flexible enough to allow many values...
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3' exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase ! High fidelity of DNA replication is ensured by 3-5 exonuclease activity of DNA polymerases Next
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic RNA usually undergoes nuclear processing C. Polypeptides are synthesized by addition of amino acids to the amino terminus. D. The 3' end of mRNA corresponds to the carboxyl terminus of the protein. Grade 2. Which of the following A. It may be autocatalytic. B. Spliceosomes are present in organelles and nuclei C. It involves removal of exons is true regarding RNA processing? D. It...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in polymerase cannot extend a new A) It is necessary for DNA synthesis because DNA polymerase canno strand in the 3 to 5' direction. B) It proofreads bases added by DNA polymerase. C) It is a type of RNA polymerase. D) It has terminal transferase activity. 28. How many of the following statements about PCNA are TRUE? • It encircles ssDNA. • It is an...
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