factors affecting rates of enzyme-catalyzed reactions; identify and describe
Ans. Enzyme catalyzed reactions are affected by many factors. The main factors affecting the rate of these reactions are temperature, pH, inhibitor, concentration of the substarte etc.
Temperature:- Velocity of the enzymatic reaction increases upto certain optimum temperature. Enzymes generally function in a narrow range of temperature. Each enzyme show its highest activity at the optimum temperature. As a rule , a typical reaction rate approximtely doubles for every 10 degree celcious rise in temperature but upto a certain value of temperature. After this temperature , the enzymes get denaturated and enzymes activity get lost. Thus both high and low temperature effects the enzymes activity.
pH :- Enzymes are very sensitive to the pH. Most enzymes are active within only a narrow range, typically 5 to 9. This pH dependance is due to the presence of the charged amino acid at the active sites. Variations in the pH cause changes in overall changes in overall charge of amino acids. And catalytic activity depends upons the specific ionization state of the amino acids. Hence pH effects the rate of reaction .
Inhibitors :- These also decrease the rates of the enzymatics reactions. These are of different types.
factors affecting rates of enzyme-catalyzed reactions; identify and describe
List 3 factors that affect the reaction rates of enzyme catalyzed physiological reactions.
Experiment 6: Factors affecting enzyme catalyzed reactions Data Page Name: 1. STANDARD CURVE Table 1: Standard Curve Use your standard curve to calculate molar absorptivity Tube# Abs. mg/ml PNP coefficient of PNP? Show work. 1 0 2 0 :183 0.063 3 0.264 0.125 4 0.530 0.25 5 0.95 0.5 Pape 57
7. Biological redox reactions are catalyzed by enzymes requiring stoichiometric co-factors. In the case of the oxidation shown below, the cofactor is derived from the vitamin riboflavin. Draw a mechanism for the reaction shown in the scheme. enzyme O FAD FADH2 enzyme succinate dehydrogenase 7. Biological redox reactions are catalyzed by enzymes requiring stoichiometric co-factors. In the case of the oxidation shown below, the cofactor is derived from the vitamin riboflavin. Draw a mechanism for the reaction shown in the...
Uncatalyzed Catalyzed Enzyme-substrate Complex In the above reaction, the lower curve is an enzyme-catalyzed reaction where the activation energy is notably lower than the uncatalyzed reaction. Suppose the enzyme in the diagram was mutated in such a way that its affinity for the substrate increased 100 fold, thereby affecting the enzyme-substrate complex portion of the curve. Assume that there was no other effect. Would you expect the reaction rate catalyzed by the altered enzyme to be faster, slower, or equal...
1-What are the factors that affect enzyme activity? 2-How the present of denaturation affecting enzyme activity? 3-Explain how the excess of H2O2inhibit the enzyme?
The Lineweaver-Burk plots shown below are for enzyme catalyzed reactions. The reaction without and inhibitor is shown in blue. The reaction with an inhibitor is shown in red. Identify the type of inhibition in each plot. with I with I 1/vo without I without I 1/[S] 1/[S] with without I without I 1/[S] 1/[S] with I without I 1/[S] Problem 4 For each plot above describe how Km and Vmax are affected by the inhibitor.
Give examples of three enzyme-catalyzed reactions and discuss each one.
List and describe the factors that can affect enzymatic reactions. a. Substrate Concentration b. Enzyme Concentration c. pH d. Temperature e. Cofactors f. Inhibitors (I have them listed but do not understand their affects)
1. The kinetics of two parallel enzyme-catalyzed reactions r'1 is expressed as aS dS2 with rı and r2 mol/L.h being the volume-specific reaction rates that include enzyme loading mE/V. The reactions are conducted in a steady state continuous flow well-mixed reactor. Deduce that the mass balances for Sı and S2 are U0 For b 20 M-1, c- 30 M-1, aT-32, dr50, S1o-1 M, and S20.12 M, calculate Si and S20 (M-mol/L). H.9520
4. (10 pts) Several factors aflecet the rates of chemical reactions. Thehemic torstsc have on rates can usually be explained using the collision thNa ffects a) Briefly describe the collision theory of chemical reactions to explaint affect the rate of chemical reactions and use collision theory factors have on the rate of reactions. The a feet these factors ur factors that fects t to exp