Question

for the following questions please indicate which answer is correct and a brief explanation why.

-

48) When attempting to determine viable cell counts of a heat-sensitive bacterial isolate, which cell counting method should be avoided?

a) spread-plate method.

b) pour-plate method.

c) live staining and direct counting w/ a microscope.

d) both spread- and pour-plate methods.

-

50) In bacterial cell undergoing binary fission and balanced growth, __________.

a) the proportion of DNA in the cell increases rapidly until the cell divides.

b) the time it takes to complete one round of DNA replication limits the generation time .

c) the proportion of protein in the cell increases as the Fts proteins form the divisome prior to cell division.

d) the proportion of all cell constituents will remain the same over time.

Answer 1

48.

The correct answer is pour plate method.   we cannot count the colony number in pour plate method because the colonies are found inside the gel but in spreading technique we just apply on the top of the gel.  

50). Answet is option B

the proportion of all cell constituents will remain the same over time.

Because the one cell divided into two, that means the two new offsprings share the parental body but not synthesize new parts.