If we flatten a circular plasmid of 105 bp with a twist number 12 how it...
If we flatten a circular plasmid of 105 bp with a twist number 12 how it will be the writhing number when we relax the plasmid to 10.5 bp per turn?
Homework Answers
Answer :)
To calculate the writhing number, we use the following formula:
Writhing number = Linking number – Twisting number
Linking number = DNA length ÷ Number of base-pair per turn
DNA length = 105 bp
Number of base-pair per turn = 10.5
Hence,
Linking number = 105 ÷ 10.5
Linking number = 10
Twisting number = 12
Therefore,
Writhing number = Linking number – Twisting number
Writhing number = 10 – 12
Writhing number = –2
Add Answer to:
If we flatten a circular plasmid of 105 bp with a twist number
12 how it...
12. When the helix axis of a closed circular duplex DNA of 2310 bp is constrained to lie in a plane, the DNA has a twist (T) of 207. When released, the DNA takes up its normal twist of 10.5 bp per tu...
12. When the helix axis of a closed circular duplex DNA of 2310 bp is constrained to lie in a plane, the DNA has a twist (T) of 207. When released, the DNA takes up its normal twist of 10.5 bp per turn. Indicate the values of the linking number (L), writhing number (W), and the twist for both the constrained and unconstrained conformational states of this DNA circle. What is the superhelix density, ơ W/T, of both the unconstrained...
can someone explain how they calculated twist T=-60 for 940 turns? U L LUIC llave superhelix...
can
someone explain how they calculated twist T=-60 for 940 turns?
U L LUIC llave superhelix densities of about 0.06. To get an idea of what this means, consider a hypothetical DNA mol- ecule of 10,500 bp, which is in the solution B form, with 10.5 bp/turn. Then L, is 10,500 bp/(10.5 bp/turn), or 1000 turns. Each DNA strand crosses the other 1000 times in the relaxed circle. If the topoisomerase DNA gyrase (see page 1055) twisted the molecule to...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see on the gel? 2. How would you estimate the total number of base pairs in a plasmid by looking at the DNA fragments of the digested plasmid on a gel? 3. If a linear 1kb DNA fragment has a restriction site that is located 50 bp from one end of the plasmid, what would you expect to see if the digested and undigested DNA...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
2. Suppose we are interested in the relationship between number of hours of exercise per week...
2. Suppose we are interested in the relationship between number of hours of exercise per week and systolic blood pressure in males 50 years of age. A random sample of 10 males 50 years of age is selected. In this analysis, the IV is number of hours of exercise per week and the DV is systolic blood pressure. The data are as follows: X = #hrs of exercise WkY=systolic BPXY 120 4 10 3 3 1 2 2 110 120...
How much heat is released when 15g of ice is heated from -12 °C to water vapor at 105 °C? (AH fus = 79.5 cal/g, AH...
How much heat is released when 15g of ice is heated from -12 °C to water vapor at 105 °C? (AH fus = 79.5 cal/g, AH vap = 539 cal/g) Substance Specific Heat (cal/g °C) 1.00 Water (liquid) Water (gas) Water (solid) 0.497 0.490
< 10 B #105 Elasticity concepts a... 1. In Modules 8 and 9 we discussed the...
< 10 B #105 Elasticity concepts a... 1. In Modules 8 and 9 we discussed the concept of price elasticity'---that is, why some Demand curves are price 'inelastic' while other Demand curves are price elastic'. 1. What are the forces that determine why some Demand curves are elastic while others are inelastic"? 2. Why is the Demand curve for gasoline considered to be inelastic while the Demand curve for one type of 'typical' new car is considered to be elastic"?...
One example of computer-aided design (CAD) is building geometric structures inter- actively. In Chapter 4, we...
One example of computer-aided design (CAD) is building geometric structures inter- actively. In Chapter 4, we will look at ways in which we can model geometric objects comprised of polygons. Here, we want to examine the interactive part. Let’s start by writing an application that will let the user specify a series of axis- aligned rectangles interactively. Each rectangle can be defined by two mouse positions at diagonally opposite corners. Consider the event listener canvas.addEventListener("mousedown", function() { gl.bindBuffer(gl.ARRAY_BUFFER, vBuffer); if...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
#8 please 7. An oxygen tank in a laboratory has an internal pressure of 12 atm...
#8 please
7. An oxygen tank in a laboratory has an internal pressure of 12 atm at 25°C. If the pressure inside the tank exceeds 25 atm, the tank will explode. A fire breaks out in the laboratory raising the temperature of the gas cylinder to 398 "C. Show how to determine and then calculate the final internal pressure of the tank and indicate if the tank will explode or is safe may assume that the volume of the tank...
12. When the helix axis of a closed circular duplex DNA of 2310 bp is constrained to lie in a plane, the DNA has a twist (T) of 207. When released, the DNA takes up its normal twist of 10.5 bp per turn. Indicate the values of the linking number (L), writhing number (W), and the twist for both the constrained and unconstrained conformational states of this DNA circle. What is the superhelix density, ơ W/T, of both the unconstrained...
can
someone explain how they calculated twist T=-60 for 940 turns?
U L LUIC llave superhelix densities of about 0.06. To get an idea of what this means, consider a hypothetical DNA mol- ecule of 10,500 bp, which is in the solution B form, with 10.5 bp/turn. Then L, is 10,500 bp/(10.5 bp/turn), or 1000 turns. Each DNA strand crosses the other 1000 times in the relaxed circle. If the topoisomerase DNA gyrase (see page 1055) twisted the molecule to...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
2. Suppose we are interested in the relationship between number of hours of exercise per week and systolic blood pressure in males 50 years of age. A random sample of 10 males 50 years of age is selected. In this analysis, the IV is number of hours of exercise per week and the DV is systolic blood pressure. The data are as follows: X = #hrs of exercise WkY=systolic BPXY 120 4 10 3 3 1 2 2 110 120...
How much heat is released when 15g of ice is heated from -12 °C to water vapor at 105 °C? (AH fus = 79.5 cal/g, AH vap = 539 cal/g) Substance Specific Heat (cal/g °C) 1.00 Water (liquid) Water (gas) Water (solid) 0.497 0.490
< 10 B #105 Elasticity concepts a... 1. In Modules 8 and 9 we discussed the concept of price elasticity'---that is, why some Demand curves are price 'inelastic' while other Demand curves are price elastic'. 1. What are the forces that determine why some Demand curves are elastic while others are inelastic"? 2. Why is the Demand curve for gasoline considered to be inelastic while the Demand curve for one type of 'typical' new car is considered to be elastic"?...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
#8 please
7. An oxygen tank in a laboratory has an internal pressure of 12 atm at 25°C. If the pressure inside the tank exceeds 25 atm, the tank will explode. A fire breaks out in the laboratory raising the temperature of the gas cylinder to 398 "C. Show how to determine and then calculate the final internal pressure of the tank and indicate if the tank will explode or is safe may assume that the volume of the tank...
Most questions answered within 3 hours.
-
Can anyone solve: "Simulation with Arena 6th Edition - Chapter 8
- Question 3E"
8-3 Change...
asked 11 minutes ago -
A 48.53 mL volume of 1.00 M HCl was mixed with 47.70 mL of 2.00
M...
asked 10 minutes ago -
Neural cell types can be specified from ESCs with
retinoic acid, conditioned medium, co-cultures or by...
asked 14 minutes ago -
What are some issues related to crimes, victims &
victimization that should be addressed?
asked 18 minutes ago -
Water flowing uniformly in a rectangular open channel has
manning value of 0.017, bottom slope of...
asked 1 hour ago -
Nature Conservancy's leader abruptly steps
down.
One morning in October 2007, Steven. J. McCormick the president...
asked 1 hour ago -
I asked a question similar to this one, which was answered
perfectly. Another practice problem is...
asked 1 hour ago -
Rachel is studying cholesterol synthesis in mice. Some mice
had a mutation in their sterol regulatory...
asked 1 hour ago -
Railco sells to its customers on account with terms of 2% / 5
/net 15. Ronco...
asked 1 hour ago -
Refer to the following lease amortization schedule. The 10
payments are made annually starting with the...
asked 1 hour ago -
Explain how God fits into Aquinas' theory of happiness.
asked 1 hour ago -
1.1 With aid of diagrams and suitable examples discuss
the economic effects of price controls.
1.2...
asked 1 hour ago