5’ctaagATGCCGATgtttaaaagACTAAAAGTTAAgtttcacagTTCAGGGCAACGGCGGTGAgtaaaa3’
3’gattcTACGGCTAcaaattttcTGATTTTCAATTcaaagtgtcAAGTCCCGTTGCCGCCACTcatttt5’
(3) Point mutations can be transition, transversion, synonymous change, and/or non-
synonymous change. What types do the following mutations belong to? For the
above DNA sequence (position index starts at 1 using the biologist’s notation),
(1). a mutation occurs at position 8, changing G to A. (2). a mutation occurs at
position 11, changing G to A. (3). a mutation occurs at position 15, changing t to
c. (4). a mutation occurs at position 12, changing A to C. (5). create a stop codon
by introducing a mutation, be it point mutation or indels (insertion/deletion). (10
points).
mRNA will be formed from 3' to 5' DNA,
5’ ctaagAUGCCGAUguuuaaaagACUAAAAGUUAAguuucacagUUCAGGGCAACGGCGGUGAguaaaa 3' (mRNA)
Removal of introns and joining of exons,
5’ AUG CCG AUA CUA AAA GUU AAU UCA GGG CAA CGG CGG UGA 3'
Amino acids formed after translation,
N met pro ile leu lys val asn ser gly gln arg arg STOP C
Now you have to perform mutations on this sequence,
mRNA,
3' ctaagAUGCCGAUguuuaaaagACUAAAAGUUAAguuucacagUUCAGGGCAACGGCGGUGAguaaaa 3'
1. G to A at 8th position,
AUG to AUA
First amino acid will change from met to ile
Mis sense mutation
2. G to A at 11th position,
CCG to CCA
pro will remain pro
No change
Silent mutation
3. T to C at 15th position
This is occuring in intron, which do not participate in translation
No change
Silent mutation
4. A to C at 12th position
CUA to CUC
leu will remain leu
No change
Silent mutation
5. Change C to G at 46th position
UCA to UGA
Ser to STOP
Please rate.
5’ctaagATGCCGATgtttaaaagACTAAAAGTTAAgtttcacagTTCAGGGCAACGGCGGTGAgtaaaa3’ 3’gattcTACGGCTAcaaattttcTGATTTTCAATTcaaagtgtcAAGTCCCGTTGCCGCCACTcatttt5’ (3) Point mutations can be transition, transversion, synonymous change, and/or non- synonymous change. What.
Exposure to various chemicals can cause DNA mutations. Sort the phrases below as transition, transversion, or insertion/deletion mutations. Note: If you answer any part of this question incorrectly, a single red X will appear indicating that you sorted one or more of the phrases incorrectly. Exposure to various chemicals can cause DNA mutations. Sort the phrases below as transition, transversion, or insertion/deletion mutations. Note: If you answer any part of this question incorrectly, a single red X will appear indicating...
Use the Mutations interactive to determine which statements describe silent mutations. The adenine of the start codon is position +1. a substitution from G to T in the arginine codon of the antisense stand a substitution of a G nucleotide at position +9 in the antisense strand a transition at position +6 in the sense strand a transversion of A in the histidine codon of the antisense strand a single nucleotide deletion at position +12 in the antisense strand a...
Mutations Worksheet-Dcletlon, Inserilon & SubstitutionThere are several types of mutations:> DELETION (a base is lost/deleted)> INSERTION (an extra base is added/inserted)- Deletion\& insertion may cause what's called a FRAMESHIFT mutation, meaning the reading "frame"changes, thus changing the amino acid sequence from this point forward > SUBSTITUTION (one base is substituted for another)- If a substitution changes the amino acid, it's called a MISSENSE mutation- If a substitution does not change the amino add, it's called a SILENT mutation- If a...
Why is an insertion or deletion more likely to result in a damaging mutation than a point mutation? 1.Point mutations only change two or three nucleotides 2.Point mutations never change the amino acid that is called for in the protein 3.Insertions/deletions change all codons that follow them, unless they are indels of 3, 6, 9, etc 4.Point mutations cannot result in a stop codon, whereas insertions and deletions can.
a) Its time to refresh your memory on the different types of DNA mutations. Define the following: • point mutation, substitution, transversion, transition, point deletion, point insertion, frameshift mutation b) Define “gene regulation” and describe why this is an important process for all cells? c) Define operon and describe the major components that exist in an operon. Why are operons most common in prokaryotes but not in eukaryotes? d) Differentiate between “inducible operon” and “repressible operon”.
*Hint: You will have one of each type. Types of Mutations? Point - Missense Frameshift - Insertion Point - Nonsense Frameshift Deletion Point - Silent Original DNA Sequence: TACACCTTGGCGACT mRNA Sequence: AUG Amino Acid Sequence: Mutated DNA Sequence #1: TACATCTTGGCGACT What's the mRNA sequence? (Circle the change) AUG TALAALLA What will be the amino acid sequence? Will there likely be effects?_ What kind of mutation is this? Mutated DNA Sequence 12: TACGAC CTTGGCGACT What's the mRNA sequence? (Circle the change)...
#1 Mutation 1. A chromosomes has the following segments, where represents the centromere: MNOPQRST Give the chromosome sequences that would result from the following mutations: a. Deletion of MN Pericentric inversion of NOP C. Inversion of RS, followed by tandem duplication of QRST b. 2. Which type(s) of chromosomal mutations: a. Increase the amount of genetic material in a particular chromosome? b. Increase the amount of genetic material in the entire genome? c. Decrease the amount of genetic material in...
Question 6 (1 point) The following DNA sequence 5'-TGCGATCC-3' is mutated to 5'-TGCGAGCC-3'. This is an example of which type of mutation? Transversion O Tautomeric shift O Insertion Transition
*Template Strand of DNA 3. The following shows the first portion of a DNA strand of a gene that is 2,500 base pairs long. AUG TACȚTCCCGGAGCCC--- TAAG LLL ODRAL a. What is the amino acid sequence encoded for by this strand starting with the T nucleotide on the left? Met b. Give an example of a synonymous substitution (a silent mutation) that could occur in the second codon of the DNA strand. c. Give an example of a nonsense mutation...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition/transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met - gin-ala - ser-val - arg - phe Mutant 1: met - gln - pro-ser -...