Question

compare similarities and differences between DNA restriction mapping and PCR

Answer 1

Similarities -

Both are very important tools of recombinant DNA technology.

Both are time consuming processes and use costly enzymes and other reagents.

Both require working with reagents taken in microlitres quantities.

Both are suseptible to even nanolitre change in volume of reagents.

Both of them require enzymes obtained from prokaryotes (taq polymerase of PCR is obtained from Thermus aquaticus and restriction enzymes used in digestion are obtained from various bacteria).

Both are temperture sensitive procedures.

Differences -

Restriction Mapping	PCR
Involves breaking down a strand of DNA into small fragments	Involves synthesizing a polymer of DNA from a small fragment of DNA
Requires temperature as low as freezing point of water	Requires high temperature
Uses restriction enzyme which breakdown DNA molecules	Uses polymerase enzyme which synthesize DNA
Requires only mixing of all reagents and incubation at low temperature	Requires mixing of all reagents and then running PCR tubes in thermocycler
At the end of the experiment, we generate a map of the chromosome showing all its restriction sites at appropriate distance.	At the end of the process, we get an amplified copy of DNA which can be used for further processes like gel electrophoresis, sequencing etc.

Please rate.