How do you create a standard calibration set with 100-µg/mL standard vials of analytes for HPLC....
How do you create a standard calibration set with 100-µg/mL standard vials of analytes for HPLC. Show your serial dilution calculation and describe your procedure
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How do you create a standard calibration set with 100-µg/mL
standard vials of analytes for HPLC....
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004...
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004 0802 esos 0.990 Show example calculation for Fe concentration of standard number 1: 5 L Use Excel to construct the calibration curve. Include the regression line and equation. Submit with your report Mass of unknown Fe sample_0.1026 Sample unknown mass diluted to 50 mL Trial Sample solution Absorbance % Fein sample per trial (mL) Dilution Volume ( 50 50 50 21 31 0,532 6...
Please explain how to go from 15 drops/L to 15 drops/mL. And show all work and...
Please explain how to go from 15 drops/L to 15 drops/mL. And
show all work and show how the final answer has to be in drops/mL
thank you
4. A student prepared a set of solutions using the serial dilution method. The stock solution had a concentration of 15 drops/L. The solutions were prepared according the following procedure. Calculation the concentrations of solutions A-D. Show the calculation for each solution, complete with equations, units, and the correct number of significant...
How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an...
How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an A260 of 1.0 Can you explain the difference?
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
may you help me figure out how to solve 7-9 Standard plate count. Include formulas and...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
You need to calibrate an instrument by creating four ~100 mL standardized stock solutions. The first...
You need to calibrate an instrument by creating four ~100 mL standardized stock solutions. The first solution will be 0.3114 M phosphoric acid. The phosphoric acid is both solid and anhydrous. The remaining three solutions will be serial dilutions of factor 3/10 from the first standard stock solution. Lay out a detailed procedure, protocol, method, and/or recipe for exactly how to create these 4 solutions including all necessary calculations and final molarities. Be sure to be specific into the exact...
You need to calibrate an instrument by creating four ~100 mL standardized stock solutions. The first...
You need to calibrate an instrument by creating four ~100 mL standardized stock solutions. The first solution will be 0.195 M copper (II) nitrite. The copper (II) nitrite is both solid and anhydrous. The remaining three solutions will be serial dilutions of factor 1/10 from the first standard stock solution. Lay out a detailed procedure, protocol, method, and/or recipe for exactly how to create these 4 solutions including all necessary calculations and final molarities. Be sure to be specific into...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
A- B- C- In Part C students will make the standard solutions to find a calibration...
A-
B-
C-
In Part C students will make the standard solutions to find a calibration curve. The experiment's Standard 1 consists of 0.13 M CuSO4. What will be the concentration of Standard 2 if it is prepared by combining 10.0 mL of Standard 1 with 10.0 mL deionized water? How should you set up the spectrophotometer? Make sure your cuvettes are clean and dry before use. Turn it on as soon as you can so it can warm up....
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004 0802 esos 0.990 Show example calculation for Fe concentration of standard number 1: 5 L Use Excel to construct the calibration curve. Include the regression line and equation. Submit with your report Mass of unknown Fe sample_0.1026 Sample unknown mass diluted to 50 mL Trial Sample solution Absorbance % Fein sample per trial (mL) Dilution Volume ( 50 50 50 21 31 0,532 6...
Please explain how to go from 15 drops/L to 15 drops/mL. And
show all work and show how the final answer has to be in drops/mL
thank you
4. A student prepared a set of solutions using the serial dilution method. The stock solution had a concentration of 15 drops/L. The solutions were prepared according the following procedure. Calculation the concentrations of solutions A-D. Show the calculation for each solution, complete with equations, units, and the correct number of significant...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
A-
B-
C-
In Part C students will make the standard solutions to find a calibration curve. The experiment's Standard 1 consists of 0.13 M CuSO4. What will be the concentration of Standard 2 if it is prepared by combining 10.0 mL of Standard 1 with 10.0 mL deionized water? How should you set up the spectrophotometer? Make sure your cuvettes are clean and dry before use. Turn it on as soon as you can so it can warm up....
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