How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an...
How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an A260 of 1.0 Can you explain the difference?
Homework Answers
The most simple and widely used method is the measurement of absorbance at 260 nm for DNA and 280 nm for protein concentration. The purines and pyrimidines in nucleic acids naturally absorb at 260 nm whereas the amino acids tyrosine, tryptophan and cysteine absorb at 280 nm.
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How do protein concentration ranges compare with the DNA value?
(DNA] of 50 µg/ml give an...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...
how do you determine DNA/RNA purity, how do you determine DNA concentration, how do you determine...
how do you determine DNA/RNA purity, how do you determine DNA concentration, how do you determine protein concentration, and how do you determine cell density?).
Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in...
Given values A260 value of the diluted sample = 0.126 Abs the concentration of DNA in my diluted sample = 6.3ug/ml Dilution factor = 20 MY QUESTION Calculate the concentration of DNA in your undiluted sample - (I got 126ng/ul as an answer but unsure if this is right) calculate the total mass of DNA you isolated *** show work for this pls
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul). A260 = 0.80...
"calculate the total mass of DNA that you isolated at the end of Part A" how...
"calculate the total mass of DNA that you isolated at the end of Part A" how would you go about doing this, the concentration in my diluted DNA sample was 6.3ug/ml, and my A60 value was 0.126 Abs i don't know if these numbers help.. part A I had to calculate the concentration of DNA in my undiluted sample to which I did Concentration (ug/mL) = (A260 – A280) dilution factor 50ug/mL C=0.126-0.060 20 50nguL= 66ng/uL and go that the concentration...
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of...
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of DNA digested per hour u to ensure complete digestion), 5X reaction butter, (b-e) to calculate how to prepare a restriction enzym a. Using the A260, what is the DNA's concentration? na miniprep and wish to digest it with EcoRI. You have DNA with an A260 of 2.0, EcoRI at digested per hour under optimal conditions, but 10 units are usually used per reaction "...
Can you pleasee answer these questions regarding the experiment of DNA quantification. What is “blank” and...
Can you pleasee answer these questions regarding the experiment of DNA quantification. What is “blank” and why do we use it in DNA quantification? If you are told to analyze DNA samples that we extracted from buccal swabs, what would you use as a blank? 3. Explain in details: a. Concentration b. A260/A280 ratio c. A260/A230 Why are those measurements used, how do you know which concentration to expect if you used the commercial kit for DNA extraction, what is...
You are a scientist working for a paternity testing center. You spent all Friday isolating dsDNA...
You are a scientist working for a paternity testing center. You spent all Friday isolating dsDNA from 100 hair samples from children and their possible dads. In order to get a quick start on your Southern Blot for Monday, you took absorbance readings for all samples using a spectrophotometer. On Monday morning you realize that you have misplaced some info for 3 of the samples. The bad news is that that spectrophotometer is broken, so you can’t take new measurements...
Calculating protein concentration: I understand that concentration is g/ml, but how can grams of protein divided...
Calculating protein concentration:
I understand that concentration is g/ml,
but how can grams of protein divided by ml of the fluid equal
percent amino acid solution? (Regarding the example calculation: 88
g / 1509 cc = 0.058 g/ml ... then how can it be 5.8% amino
acid solution??)
"Determine protein concentration.
Subtract volume of lipid from fluid requirement
to determine remaining fluid needs.
Divide protein requirement (in grams) by remaining fluid
requirement and multiply by 100. This gives you the...
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of DNA digested per hour u to ensure complete digestion), 5X reaction butter, (b-e) to calculate how to prepare a restriction enzym a. Using the A260, what is the DNA's concentration? na miniprep and wish to digest it with EcoRI. You have DNA with an A260 of 2.0, EcoRI at digested per hour under optimal conditions, but 10 units are usually used per reaction "...
Calculating protein concentration:
I understand that concentration is g/ml,
but how can grams of protein divided by ml of the fluid equal
percent amino acid solution? (Regarding the example calculation: 88
g / 1509 cc = 0.058 g/ml ... then how can it be 5.8% amino
acid solution??)
"Determine protein concentration.
Subtract volume of lipid from fluid requirement
to determine remaining fluid needs.
Divide protein requirement (in grams) by remaining fluid
requirement and multiply by 100. This gives you the...
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