The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
Homework Answers
Ans- if sample protein concentration is greater then linear range than you would need to dilute the sample.
In this question, the maximum linear range is 2 mg/ml, and your sample contains protein concentration 50mg/ml,
then you dilute your sample 100 times.
It becomes 0.5mg now; you can easily measure the protein concentration of your sample. 100 time dilution would be 99 part of your choice of buffer and 1 part of your sample.
To calculate the concentration of the undiluted, unknown sample, simply multiply by the dilution factor. 100*0.5=50 mg
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The linear concentration range for the Bradford assay is 20-2000
µg/mL of protein. Protein concentrations below...
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I forgot to put down that the observance (=OD) at 280nm is
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Please answer #2. I'm unsure how its done
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