Question

Show picture of a gel in a rig, loaded with a negative molecule, neutral molecule, and a positive molecule, show which way each will migrate.

Answer 1

Gel loaded with a negative, positive and neutral molecule

MOLECULES	CHARGE	BEHAVIOUR
DNA	Negative	Moves to positive
RNA	Negative	Moves to positive
Proteins	Positive Negative Neutral	Moves to negative Moves to positive No movement
Carbohydrates	Neutral	No movement
Lipids	Neutral	No movement

According to the above properties of the molecules, we can show the molecules position in the gel. They can be depicted as:

Hence, in the above 2 figures it has clearly mentioned the position of protein molecules loaded irrespective of positive, negative and neutral charge properties in well through the gel.