Predict what effect taxol, colchicine and cytochalasin D will have on cilia length and the actin cytoskeleton.
Predict what effect taxol, colchicine and cytochalasin D will have on cilia length and the actin...
What effect did each drug have on cell motility? a. Cytochalasin D b. Cholchicine c. Taxol
You have a 100X stock of cytochalasin (a drug that depolymerizes actin). You want to treat some cells with cytochalasin and caffeine to see what happens. The cells are already in 200μl of a solution of caffeine at approximately the right concentration. How much cytochalasin would you add to the cells in caffeine?
Question 1 20 pts Cytochalasin is a drug that caps actin filament plus ends, thus preventing actin polymerization. Phalloidin is a drug that binds to and stabilizes actin filaments, preventing actin depolymerization. Even though these drugs have opposite effects on actin polymerization, the addition of either of these drugs instantaneously freezes the cell movements that depends on actin filaments. Explain why drugs that have opposite effects on actin filaments can have a similar effect on cell movements.
Predict what would happen if you treated cells with Cytocholasin D when the concentration of G-actin is lower than the critical concentration for either end.
You have added a pure solution of formin to a solution of actin in ionic conditions appropriate for actin polymerization (i.e. 100 mM K+, 2 mM Mg2+, and 1 mM ATP). What do you predict occurs to the rate of actin filament assembly? o The rate of actin filament assembly increases only on the plus ends of the actin filaments, on the minus end it is unchanged. The rate of actin filament assembly is unchanged at the minus end and...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
can someone please answer question C :) a Why is the process of cytoplasmic streaming important in a large cell such as Nitella? b How do the inhibitors cytochalasin and oryzalin work? c If you were to repeat the experiment with an inhibitor and the DMSO control and the DMSO slowed streaming to the same rate as the inhibitor what would your conclusion be? (The experiment was an investigation into the role of the cytoskeleton and calcium ion channels in...
What effect does the length of a string have on the wavelength of the vibrations?
answer
only question number 7.please answer only question number 7
Why termination of a cell signaling event is important? Explain with two examples how GPCR signaling is terminated. What is actin filaments treadmilling? What would be the consequence for actin filament assembly/disassembly if a mutation prevented actin's ability to bind ATP? What would be the consequence if a mutation prevented actin's ability to hydrolyze ATP? Predict the effect on a cell's actin cytoskeleton if function blocking antibody against cofilin was...
Isolated bundles of actin filaments from the acrosomal processes
of Limuluspolyphemus(horseshoe crab) sperm have readily
distinguishable plus ends (tapered) and minus ends
(blunt). Assembly at the ends of such bundles was used
to determine the mechanism of action of phalloidin, which has a
marked effect on actin assembly. When phalloidin is mixed with
actin in a molar ratio of at least 1:1, the growth rate increases
at both ends, as shown for minus ends in Fig.
16-11A. Because growth rate = kon[actin]...