Please, list the general reaction that is catalysed by RNA polymerase and why is Mg2+ important? (diagrams are acceptable)
Please, list the general reaction that is catalysed by RNA polymerase and why is Mg2+ important?...
Briefly describe the reaction catalysed by DNA polymerase III. In your answer, describe the two polymerase active sites and the type of bond catalysed.
1) RNA polymerase has low affinity to mRNA – why is that? 2) Initially, RNA polymerase makes a few short transcripts, why?
Why does E. coli have several different sigma factors? A.They allow RNA polymerase I, RNA polymerase II and RNA polymerase III to bind to different promoters. B.They allow the different subunits of the RNA polymerase holoenzyme to bind to each other. C.They are redundant in case one is mutated. They all perform the same function. D.One is needed to transcribe mRNA. A second is needed to transcribe tRNA. And a third is needed to transcribe rRNA. E.They are used if...
Why are related genes transcribed together? Why is transcription regulated? RNA Polymerase does not have proofreading mechanism and the error rate is 1 per 104~105, why is fidelity not as important as during DNA replication? Which RNAs are not translated to proteins?
why is it that the DNA polymerase needs 4 deoxyribonucletides else RNA polymerase need only 2NTP
Consider the reaction that causes RNA to hydrolyze at high PH. Why is RNA more sensitive to high PH than DNA? What is the function of Mg2+ in RNA molecule?
23. Why Mg2+ is important in ATP hydrolysis reaction? 25. What is the AG" of acetyl-CoA hydrolysis?
I'm thinking of the human genome specfically, but more general answers are welcome. As RNA polymerase moves along the DNA helix it follows a single strand. The two DNA strands are unwound locally by a helicase enzyme. However, after some distance, I imagine that either the polymerase must move around the axis of the DNA OR the DNA must somehow be rotated along it's length to relieve the twisting by the polymerase. I can't quite understand how the DNA is...
The BL21(DE3) expression cells carry a λDE3 integration containing the T7-RNA polymerase gene. A) Why is the T7-RNA polymerase protein so specific with regard to the target genes it transcribes? B) Is this enzyme predicted to transcribe any E. coli genes? Why or why not?
Compared to RNA elongation by RNA polymerases, DNA elongation by DNA polymerase requires an additional reaction component. Which is appropriate for fulfillment of this requirement? Select one: a. a primase is needed for the biological DNA replication. b. a short DNA primer is necessary for PCR. c. a host tRNA is used for the synthesis of DNA by viral reverse transcriptase. d. All of these e. None of these