Briefly describe the reaction catalysed by DNA polymerase III. In your answer, describe the two polymerase active sites and the type of bond catalysed.
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Briefly describe the reaction catalysed by DNA polymerase III. In your answer, describe the two polymerase...
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in the replication of E. coli DNA. What features of the E. coli replisome and of DNA polymerase III’s structure are associated with replication processivity? How do mammalian cells prime discontinuous strand replication and how do they remove RNA primers?
Describe DNA replication on the leading and lagging strands of DNA. Your answer must include a description of the roles of the following enzymes: -DNA polymerase I (5’-3’polymerase activity, 3’-5’ exonuclease activity, 5’-3’ exonuclease activity) -DNA polymerase III (5’-3’polymerase activity, 3’-5’ exonuclease activity) -helicase, primase, ligase, gyrase
1. Describe the details of these two fundamental properties of the activity of DNA polymerase a. The direction of synthesis of the chain growth of the new strand b. What is required for DNA polymerase to add additional nucleotides?
SELLUN 9. Contrast the functions of DNA polymerase III and DNA polymerase I. 10. Why do are the start points of DNA replication in eukaryotic and prokaryotic organisms AT rich? 11. What is the start point of DNA replication called in E. coli? What is it called in humans? 12. In DNA replication in E. coli which enters the replication fork first, DnaA protein, or Helicase? 13. In eukaryotes, two proteins are involved in linking DNA replication to S phase...
Question 23 Short Answer Question 2. The enzyme DNA polymerase is used to synthesize DNA, and the enzyme RNA polymerase is used to synthesize RNA. Using the content discussed in BIO130, describe two additional differences in how these enzymes function (not including the bases used). (3 sentences maximum, 2 marks)
18. DNA polymerase III needs a to be present before it can begin copying DNA A. Promoter B. Phosphorylation Cascade C. RNA Primer D. Transcription Factor 19. This molecule is a monomer of a. RNA b. DNA c. ATP d. Protein 20. Which organelle often contains numerous ribosomes? a. Rough Endoplasmic Reticulum b. Smooth Endoplasmic Reticulum c. Golgi Apparatus d. Nucleus 21. What type of mutation would occur if CAU mutated to CAC? a. Missense b. Silent c. Major d....
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
Why are we treating our purified PCR products with a DNA polymerase before adding them to the ligation reaction? and What is the purpose of ATP in the ligation reaction? Group of answer choices -It provides energy to the ligase to catalyze a peptide bond -It provides energy to the ligase to catalyze a phosphodiester bond -It provides energy to the ligase to cut the DNA -It provides energy to the ligase to unwind the DNA
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication, replication fork, DNA gyrase, helicase, primase, DNA polymerase, DNA ligase, leading strand, lagging strand, continuous replication, non-continuous replication, and Okazaki fragment)
Mg2+ serves the following function(s) in the reaction catalyzed by DNA polymerase. Multiple answers allowed A. It stabilizes the pentacoordinate transition state of the phosphodiester bond formation. B. It precipitates inorganic phosphate (Pi) arising from the reaction. C. It interacts with the 3'-hydroxyl of the primer and a phosphate in the dNTP. D. It stabilizes the negative charge on the departing pyrophosphate which is derived from the incoming dNTP. E. It interacts with the 3' hydroxyl of the incoming dNTP.