Answer the following questions: a) What is special about the E coli BL21(DE3)pLysS cells used for...
Answer the following questions:
a) What is special about the E coli BL21(DE3)pLysS cells used for gene expression?
b) In what structural form is the expressed gdhA gene synthesized in the E coli host cells?
c) What physical attributes of SDS-PAGE gels allow the separation and molecular weight determination of polypeptides?
d) Given the size of the gdhA gene of ~1362 bp, what is the theoretical size of the expressed protein?
e) Does your answer from (d) agree with the MW size of your expressed protein, and if not, Why?
Homework Answers
The que is not clearAdd Answer to:
Answer the following questions:
a) What is special about the E coli BL21(DE3)pLysS
cells used for...
What is the mechanism of IPTG induction for pET-P1B in BL21 E. coli cells?
What is the mechanism of IPTG induction for pET-P1B in BL21 E. coli cells?
4) (2 pts) Explain what will happen to an E. coli culture in the following media:...
4) (2 pts) Explain what will happen to an E. coli culture in the following media: a. No glucose + no lactose (1 pt) b. Glucose + lactose but the cells have a supper repressor mutation (1 pt) 5) The lacZ gene codes for a protein in the lac operon. What is this protein and name its function? (2 pts)
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation...
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation of the protein is necessary so that proteins run proportionally to their size, based on the interaction of SDS with the unfolded protein. Heat is used to hydrolyze the peptide bonds of the protein. The heat step allows the proteins to unfold, enabling the protein chain to be coated with SDS molecules. Heat is used to hydrolyze disulfide bonds. QUESTION 2 1.5 points Saved...
Answer the following questions that do not have an answer. Creating a graph is not necessary....
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
after the pGlo vector was...
8. DNA cloning. The first DNA clone using recombinant DNA technology was human insulin, which was...
8. DNA cloning. The first DNA clone using recombinant DNA technology was human insulin, which was made in 1978. The insulin gene was inserted into a plasmid and the gene was transcribed and translated from the plasmid in E coll cells. Insulin is synthesized by ribosomes in human pancreatic cells. Insulin is synthesized as a longer polypeptide, which is then trimmed by proteases to make the mature chains A and B (see Fig. 2.17 on page 39 of your textbook)....
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
8. (6 pts) E. coli is a facultative anaerobe. Using the data in the table below, answer the following questions: a. What type of experiment or technique was used b. What is the doubling time in e...
8. (6 pts) E. coli is a facultative anaerobe. Using the data in the table below, answer the following questions: a. What type of experiment or technique was used b. What is the doubling time in each growth c. What is the reason for the difference in doubling to calculate CFU/ml? condition? time? Terminal electron acceptor CFU/ml at time 0 CFU/ml at time 8 hrs O2 1.25 x 102 1.25 x 102 7.5 x 108 3.1 x 106 NO3
8....
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
answer all the questions 1) All of the following contribute to promoter binding by RNA polymerase...
answer all the questions
1) All of the following contribute to promoter binding by RNA polymerase I in bacteria except: a)-10 consensus sequence b)-35 consensus sequence c) rho factor d) sigma factor e) none of the above 2) Common structural changes or lesions found in DNA after exposure to ultraviolet light are: a) thymine dimers b) cytosine dimers c) purine dimers d) adenine dimers e) none of the above 3) What is the function of the sigma subunit in the...
Why are proteins heat denatured prior to analysis in SDS-PAGE? Select all answers that apply. Denaturation of the protein is necessary so that proteins run proportionally to their size, based on the interaction of SDS with the unfolded protein. Heat is used to hydrolyze the peptide bonds of the protein. The heat step allows the proteins to unfold, enabling the protein chain to be coated with SDS molecules. Heat is used to hydrolyze disulfide bonds. QUESTION 2 1.5 points Saved...
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
after the pGlo vector was...
8. DNA cloning. The first DNA clone using recombinant DNA technology was human insulin, which was made in 1978. The insulin gene was inserted into a plasmid and the gene was transcribed and translated from the plasmid in E coll cells. Insulin is synthesized by ribosomes in human pancreatic cells. Insulin is synthesized as a longer polypeptide, which is then trimmed by proteases to make the mature chains A and B (see Fig. 2.17 on page 39 of your textbook)....
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
8. (6 pts) E. coli is a facultative anaerobe. Using the data in the table below, answer the following questions: a. What type of experiment or technique was used b. What is the doubling time in each growth c. What is the reason for the difference in doubling to calculate CFU/ml? condition? time? Terminal electron acceptor CFU/ml at time 0 CFU/ml at time 8 hrs O2 1.25 x 102 1.25 x 102 7.5 x 108 3.1 x 106 NO3
8....
answer all the questions
1) All of the following contribute to promoter binding by RNA polymerase I in bacteria except: a)-10 consensus sequence b)-35 consensus sequence c) rho factor d) sigma factor e) none of the above 2) Common structural changes or lesions found in DNA after exposure to ultraviolet light are: a) thymine dimers b) cytosine dimers c) purine dimers d) adenine dimers e) none of the above 3) What is the function of the sigma subunit in the...
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