How can you make RT-PCR gives you an accurate reading about the amount of the target?...
How can you make RT-PCR gives you an accurate reading about the amount of the target? target gene?
Homework Answers
RT-PCR or the real time Polymerase chain reaction helps in determining the rate of PCR in real time. It is because the template DNA is added with some fluorescence tags. So, as the amplification proceeds and the number of cycle increases, the fluorescence signal also keeps increasing which is taken as an indicative of increasing amplification. Then increasing amplification automatically corresponds to the increasing amounts of target/target gene.
Hope it helps, Good luck !!!!!!
PLEASE DO PRESS LIKE :)
Add Answer to:
How can you make RT-PCR gives you an accurate reading about the
amount of the target?...
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount...
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR...
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR...
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken. CRISPR is less expensive than PCR Question 5 4 pts What are some differences between the PCR that occurs in the Sherlock protocol and the...
D Question 3 2 pts When and how does the RT-PCR test detect the presence of...
D Question 3 2 pts When and how does the RT-PCR test detect the presence of the virus? After PCR, by adding a quencher dye which is activated when the viral DNA is available in high concentrations During PCR by adding fluorescent primers that are detected through a special laser After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding a fluorescent dye and a quencher dye...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast...
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique(s) could she use and why?
Today we amplified 50 ng of Bos taurus (calf) DNA by PCR. This amount of DNA...
Today we amplified 50 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 15,000 molecules of the insulin gene [50 ng DNA= 2.5 x 10-20mol; (2.5x10-20)x(6.023×1023) = 1.5 x 104molecules]. We performed PCR for 35 cycles to amplify the amount of this gene. a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2Nformula)? b. How many molecules of the insulin gene would, therefore, be present after PCR?
How can reading about the illness that others experience help us make sense of the tragedy...
How can reading about the illness that others experience help us make sense of the tragedy and injustice in the world?
Assume that you are using qPCR to determine the amount of DNA in a given sample....
Assume that you are using qPCR to determine the amount of DNA in a given sample. Sample 1, from which you know that it contained an amount of 1 pg, required 15 PCR cycles to reach a specific level of PCR product. Your sample 2 required only 5 PCR cycles to reach the same level. Sample 3 required 18 PCR cycles to come to the same level of PCR product. How much DNA did samples 2 and 3 contain? RT-qPCR...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The test requires a lab to run the PCR Collateral RNAse activity is activated when RNA guide finds the target RNA (COVID19). If the Reporter RNA is cleaved by CAS13 it binds to the test strip, 2 lines will appear test positive The Cas13 protein attached to the RNA guide has collateral RNA activity This test does not require a PCR step Question 2 2...
Based on this question can you give me examples how PCR can be used for biotechology?...
Based on this question can you give me examples how PCR can be used for biotechology? How can PCR be a useful tool for biotechnology? -to make multiple copies of a piece of DNA enzymatically -used to: -clone DNA for recombination -amplify DNA to detectable levels -sequence DNA -diagnose genetic disease -detect pathogens
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR...
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken. CRISPR is less expensive than PCR Question 5 4 pts What are some differences between the PCR that occurs in the Sherlock protocol and the...
D Question 3 2 pts When and how does the RT-PCR test detect the presence of the virus? After PCR, by adding a quencher dye which is activated when the viral DNA is available in high concentrations During PCR by adding fluorescent primers that are detected through a special laser After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding a fluorescent dye and a quencher dye...
When and how does the RT-PCR test detect the presence of the virus? During PCR. by adding a fluorescent dye and a quencher dye which will be degraded if DNA polymerase finds the viral gene. The sample if positive fluoresces. After PCR, by adding a fluorescent dye which will be activated once the sample goes through a special laser. During PCR, by adding fluorescent primers that are detected through a special laser. After PCR, by adding a quencher dye which...
Question 1 6 pts Which of the following statements are true about the Sherlock protocol: The test requires a lab to run the PCR Collateral RNAse activity is activated when RNA guide finds the target RNA (COVID19). If the Reporter RNA is cleaved by CAS13 it binds to the test strip, 2 lines will appear test positive The Cas13 protein attached to the RNA guide has collateral RNA activity This test does not require a PCR step Question 2 2...
Most questions answered within 3 hours.
-
1. Are all good samples random?
2. Magazines often report surveys giving statistics such as “63%...
asked 19 minutes ago -
Under all the various types of market structures, firms
must eventually earn some economic profits for...
asked 6 minutes ago -
Consider the following fitness regime for a single locus trait
with two co-dominant alleles: w11 =...
asked 10 minutes ago -
A large cable company reports the following.
80% of its customers subscribe to its cable TV...
asked 26 minutes ago -
Please answer the question in brief.
Discuss the role of ERP in organizations. Are ERP tools...
asked 12 minutes ago -
Discuss the pros and cons of collaborative software such
as SameTime. Does it increase productivity? What...
asked 24 minutes ago -
Buying your in-laws a gift because it’s expected is
due to the ____________ motive of gift-giving....
asked 27 minutes ago -
Calculate the expected value, the variance, and the standard
deviation of the given random variable X....
asked 1 hour ago -
A hospital performs 100 surgeries per week. The probability that
complications after surgery occur is 10%....
asked 1 hour ago -
1 point) Given the significance level α=0.01 find the following:
(a) left-tailed z value z= (b)...
asked 1 hour ago -
Assuming you are the head of the software development unit at
Cyber.Soft, explain and justify why...
asked 35 minutes ago -
Magnesium and nitrogen react in a combination reaction to
produce magnesium nitride. 3 Mg + N2...
asked 43 minutes ago