Question

1- Are materials and plates opened in the back of the laminar flow hood more or less likely to get contaminated during your work session? (select one choice) And why? the options either less or more

2- Is everything placed in a laminar flow hood sterile?

3- You reused the micropipette tip used for dispersing the cell pellet to withdraw media from your stock media. What should you do now?

4- How can you detect contamination in your culture? What should you do if you detect contamination?

Answer 1

1.

The air at the back is pure. The air in the front is bit contaminated.

So, when the plate is opened at the back will be sterile. So, the plate at the back will get less contaminated.

2. Liquid media cannot be sterile in laminar flow. Objects can be sterile.

3. We can either autoclave the media again. If we see growth then we will have to discard the media

4. If we see filamentous growth and turbidity in the culture then it means that it is contaminated. If contamination is there then we have to do the isolation of the bacteria of our choice if it is also not possible we can do isolation by plating the organism on antibiotic plate.