If the volume of DNA solution was 0.5 mL, calculate the yield if:
(a) the Absorbance reading at 260nm from a 1:100 dilution = 0.459
(b) the Absorbance reading at 260nm from a 1:100 dilution = 0.167
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Ans: The volume of DNA solution was 0.5 mL.
(a) DNA concentration (µg/ml) = OD of DNA at 260nm × dilution factor × 50µg/ml
= 0.459 x 1/100 x 50
= 0.2295 µg/ml
DNA yield (µg) = DNA concentration × total sample volume (ml)
= 0.2295 x 0.5
= 0.1147 µg
(b) DNA concentration (µg/ml) = OD of DNA at 260nm × dilution factor × 50µg/ml
= 0.167 x 1/100 x 50
= 0.0835 µg/ml
DNA yield (µg) = DNA concentration × total sample volume (ml)
= 0.0835 x 0.5
= 0.0417 µg
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