Question

One method of measuring DNA is to use a spectrophotometer. A DNA solution absorbs light of...

One method of measuring DNA is to use a spectrophotometer. A DNA solution absorbs light of 260nm in proportion to concentration so that an optical density OD260 of 1 is equivalent to 50µg of DNA in 1mL of H2O. A student places 3µL of a DNA solution into a cuvette containing 997µL of H2O and obtains an OD260 of 0.034. What is the concentration of the original DNA sample in mg/mL? Please provide your answer to three decimal places and show your calculations.

0 0
Add a comment Improve this question Transcribed image text
Know the answer?
Add Answer to:
One method of measuring DNA is to use a spectrophotometer. A DNA solution absorbs light of...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...

    One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...

  • 18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...

    18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...

  • PreLab Assignment for Measuring Manganese Using Spectrophotometry List health effects associated with the of exc manganese...

    PreLab Assignment for Measuring Manganese Using Spectrophotometry List health effects associated with the of exc manganese abortion per te mount of h isproportional to what we factors worted by a gre a t w Suppose you need to do option to experiment a olion. Approximately what was also would be reworst m ance what would be the best wavelength of light to measure the Why? Explan your answers in one or mor e g end e wrong w Best wavelength...

  • help please? this was the only other information given REPORT SHEET Determination of the Solubility-Product Constant...

    help please? this was the only other information given REPORT SHEET Determination of the Solubility-Product Constant for a Sparingly Soluble Salt EXPERIMENT 8 A. Preparation of a Calibration Curve Initial (Cro121 0.0024 M Absorbance 5 mL Volume of 0.0024 M K Cro Total volume 1. I mL 100 mL 2. 100ML 3. 10 mL 100ml 4. 15 mL 100 ML Molar extinction coefficient for [CrO2) [Cro,2) 2.4x100M 12x1044 2.4810M 3.6810M 0.04) 2037.37 0.85 1.13 2. 3. Average molar extinction coefficient...

  • Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...

    Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...

  • Food and beverage containers are commonly made of tin, steel, aluminum, or a combination of metals....

    Food and beverage containers are commonly made of tin, steel, aluminum, or a combination of metals. Suppose that a laboratory procedure requires a can be made of aluminum. Which property can be used to determine if a can is likely to be made of aluminum? O color Odensity volume mass What is the density of a pear that occupies 32 cm and has a mass of 42 g O 0.7619 g-cm 0 2.0 g.cm O 1.3125 g-cm O 0.76 g.cm...

  • 1) What chemical is the titrant in this experiment? What chemical is the analyte in this experiment? Experi...

    1) What chemical is the titrant in this experiment? What chemical is the analyte in this experiment? Experiment #8: Measuring the Vitamin C Content of Emergen-C™ Objectives: • Students will learn how to use a burette. • Students will learn how to use the iodine starch indicator system to monitor oxidation/reduction reactions. Students will learn how to conduct a titration to determine the amount of analyte in an unknown solution. • Students will learn about ascorbic acid's role as a...

  • For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2

    For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2....the problem is that if I don't boil it for that long and (30 min) b/c of not enough time but I put the water I boiled for 1/2 hr aproximately into a NaOH bottle with a CO2 absorber and stored it there for a few days. I would assume that I would have to boil the water again...but...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT