Briefly describe how cells determine whether or not to express genes in the his operon. Be sure to include the mechanism for why expression occurs or does not occur.
The operon is a functional unit of get containing a cluster of
genes. These genes are controlled by single promotor , and
operator. Most of the time the gene contained in the operon are the
genes usually expressed at the same time. suppose if the gene
corresponding to an antibiotic resistance or the heat shock protein
are to be expressed at a certain time. As these genes are not
needed all the time the repressor protein is bound to the operator
region and the polymerase could not bind to the promotor. Which
allows the genes not to be expressed. When there is a need of those
gene to be expressed. The repressor protein is released from the
operator region by different means (phosphorilation /
dephosphorilation / presence of certain compound) and the
successful expression of gene happens.
Ex: Like in Lac operon the repressor protein is bound to the
operator region and the presence of allolactose or biosimilar
compound like IPTG removes the repressor protein and carries the
successful transcription of gene and gene expression.
Briefly describe how cells determine whether or not to express genes in the his operon. Be...
4. Briefly draw and/or describe the functions of the genes contained in the lac operon. Why is a low level of lac operon expression necessary for it to respond to the presence of lactose ?
Describe in detail two ways in which transcription of the trp operon (containing genes used for biosynthesis of trypotophan) and lac operon (containing genes used for digestion of lactose) are regulated differently. Include what types of proteins/ligands are regulating each operon, and how the various components interact. Diagrams may be helpful to your explanation.
For this assignment you will complete an illustration(s) (by hand) of how the lac operon works. You should be sure to include details in your drawing such as the names of relevant structural genes (lac Z and lac Y) and controlling sequences (operator, promoter, repressor). 1. Show the influence of lactose (in the form of allolactose) on the repressor protein. 2. Show what effect the primary structural genes have on lactose. 3. Show how glucose levels can influence the expression...
Yet, all the cells in your body contain the same genes (and same alleles). The difference across cell types is that genes get selectively expressed (turned on or off) based on the proteins needed for cellular function given their environment. Select which statement explains the reason why hair does not normally grow on your muscle cells. a. Muscle cells have the gene for keratin, but do not express it b. Muscle cells do not have the gene for keratin and...
1. Describe how bacteria regulate genes through operons. 2. What is the difference between a repressible and inducible genetic system? 3. Expression of the Lac operon is under the controls of (1) the Lacl repressor and (2) CAP. Consider each of the following conditions and determine which factors will be acting on the operon. Predict the rate of gene expression. Conditions Lac Operon fon" or "off" CAP present" or "absent" at Lac Operon Predict the rate of gene expression (none,...
1. Describe in detail how both positive and negative regulation control expression of the lac operon in bacteria. In doing this, also convey understanding of how and why expression of this operon is controlled two ways (positive and negative). Also answer, what are the energetic benefits to the cell of operon regulation? 2. What is horizontal gene transfer? Describe in detail the three mechanisms of horizontal gene transfer. What are the evolutionary advantages and costs of horizontal gene transfer. I...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
What is DNA methylation? Describe how this mechanism regulates the expression of genes. What can happen when the DNA is hyper-methylated? Pick and describe a disease that results from DNA hyper-methylation.
Please list and briefly describe some differences between bacterial and animal cells that have not been targeted with antibiotics. For each one you list indicate whether you think this could be a target and why.
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...