Question

urea hydrololysis

suppose you ran this test with Providencia stuartii,but it took 48 hours to turn pink.Do you think this is a false result? If so, is it a false positive or falsenegative? if not, why not? Give some possible explanations for this occurence.
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Answer #1

Yes it is a false positive reaction.

Urease production is indicated by a bright pink color on the slant indicating hydrolysis of urea which should occur in 24 hours of incubation, but as it take 48 hours to show pink colour indicating hydrolysis of proteins in the medium rather than urea hydrolysis.

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