Question

QUESTION 7 For the following reaction choose the correct rate expression in terms of the appearance of one of the 2H20 (g)2H29)+ 02g) Ob 1/2&H21/ Click Save and Submit to saove and submzlick Save All Ansvers to save all answers. bnmite Cliek Save All nswers to save all onswers Save All Answers 19 Restart On 4:19 1 Snapchat X Class Exercise Gel Elecreophoresis... Class Exercise: Gel-ElectrophoresisBio 105 Name http://learn.genetics.utah.edu/content/labs/gell 1. What is the definition Agarose Gel Electrophoresis? (U may use lab manual) Where it is used? 2. Can the agarose gel used for protein separation? If not, why? 3. What are chemicals required to prepare the gel? 4. What is the required apparatus to make a gel? 5. What is the purpose of adding loading dye? 6. DNA is loaded on which side? Cathode or Anode? Why it is loaded on that side? 7. What is the purpose of DNA marker 8. Name the apparatus that is required to view the gel? 9. What will happen of you orient the gel in opposite way than the one shown in the animation 10. Two proteins of size 30 k and 12.5 k. Which one will run faster and Why?

Answer 1

Question 7 : answer is option b

1.Agarose gel electrophoresis is used for DNA separation.

2.Agarose gels are usually used for DNA and the pores of the gel are too big to have a good separation for proteins.

3.Chemicals used are Agarose and Tris acetate EDTA buffer.

4.Gel casting Tray.

5.Loading dye helps to see the dye front on the agarose gel.

7.Purpose of DNA marker is to compare the sample with known standards.

8.Apparatus need is UV trans-illuminator.

Lower size will run fast so obviously 12.5 k will be run fast.