Question

5. (4 pts) In an immunocytochemical experiment you use a primary antibody against a presy What is your interpretation of this result and what control experiment could you do to determine if the antibody only labels the target protein and not any other off-target protein(s)? (Hint: this control experiment cannot be done in crayfish but only in animals that can be genetically modified, e.g. mouse, Drosophila) naptic protein and you get labeling in an unexpected location (neuronal nuclei).

Answer 1

You must understand that immunology experiments with antibodies is usually to use a purified protein since a cellular culture. If we suppose this process, the error could be by a bad process of purified, there are: i) Contamination of presynaptic protein with neuronal proteins, ii) Error process of purified in that is separated other protein than the presynaptic protein, iii) Other process errors. The most simple animal to experiment is in mouse, and you inject directly to their brains and revel with fluorophore markers.