Question

#4 the reagnet is HCl

3. Look at your plot of the data. Which of the four reagents was the most effective at denaturing protein-chromophore complex? Justify your answer. 12 pts] e lon Hc1 was the most effective. This is because it is aston a cid, whrin esuited in the qrcatest ratt of denatoratianc to the othex denaturants. Consider the chemical chain might be chemically changed when the reagent identifned in #3 ts added to the protein. Write properties of this reagent. Identify by name an amino acid residue whose side the chemical equation for the chemical reaction that occurs when said reagent reacts with the side chain functional group of this specific amino acid. Remember that the control is dissolved in pH 7 phosphate buffer. [4 pts] 5. Which non-covalent interaction listed on p.37 is most negatively affected when the reaction in #4 occurs in a protein? [1 pt] 6. Briefly explain why HCI and HC,H 02 (acetic acid) affect the protein-chromophore complex differently even though they are both acids. Be specific in your answer. [2 pts] Hel is a strong acidよdisassociates almost completely when acting Complex. 7. Consider the chemical properties of Hydrochloric acid. Identify by name an amino acid resid whose side chain might be chemically changed when HCI is added to the protein. Write the c uation for the chemical reaction that occurs when HCI reacts with the side chain functional ic dismlyed in pH 7 phosphate buffer

Answer 1

6) Hi9 a sbrong acid and Can break -th e complex Lompletely, probobly by denatuwing the protein. Acetic ads a weak acid and Can act as a buffer, thus it can change the p of the Solcthon, therefore t changes the properties of the chromophore complex, Such as Wavelerg anje lerqth maxima, Color change