Question

A Mutation in the Myostatin Gene Increases Muscle Mass and Enhances Racing Performance in Heterozygote Dogs Assignment 2 Below are the sequences of wildtype and mutant alleles of the myostatin gene discussed in the paper introduction Wildtype Allele: AATTACTGCTCTGGAGAGTGTGAATTTGTG Mutant Allele: AATTACTGCTCTGGAGAGTGAATTTGTGTT 1) Using the genetic code table (provided on page 2) and single-letter code for amino acids, write the amino acid sequences of both predicted proteins 2) What might have happened, to generate the mutant allele? 3) How does the wildtype protein compare to the mutant protein? Produce a sketch that compares them. 4) In dogs (which are diploid), the myostatin gene is on chromosome 37 Sketch the myostatin alleles found on chromosome 37 for each of the following A heterozygous whippet A wildtype whippet A homozygous mutant whippet 5) Given the differences between the normal and mutant proteins, and the phenotypes seen (as described in the introduction that you concept mapped), suggest and illustrate (in simple diagrammatic sketches) two different possible explanations for why the mutant protein functions differently than the wildtype protein. Add a short (1 or 2 sentence) caption to each sketch.

Can someone please help me with numbers 4 and 5?

Answer 1

ANSWER FOR 4

DIPLOID MEANS THE COMPLETE SET OF TWO GENES WHICH ARE PASSED FROM PARENTS.THE ONE COPY OF THE GENE FROM FATHER AND OTHER FROM MOTHER.HERE THEY ASKED WHICH ARE DIPLOID.IN WHIPPET DOGS SOMATIC CELLS ARE IN DIPLOID CONDITION.AND THE EGG AND SPERM ARE HAPLOID. SO HERE THE POSSIBLE ALL ARE DIPLOID CONDITION ONE CAME FROM FATHER AND OTHER FROM MOTHER

ANSWER 5

MYOSTATIN IS A PROTEIN RESPONSIBLE FOR INHIBITION OF MYOGENESIS AND DIFFERENTIATION,IN THIS CONDITION THE MUTANT GENE CONTAINS STOP CODON WHICH PRODUCE A PREMATURE TRUNCATED PROTEIN LEADS LOST ITS ORIGINAL FUNCTION.

THE LOSS OF ACTIVE SITE IN THIS PROTEIN MIGHT BE THE REASON FOR MYOGENESIS.

WILD TYPE

DNA   5' AAT TAC TGC TCT GGA GAG TGT GAA TTT GTG 3'

RNA 3' UUA AUG ACG UGU CCU CUC ACA CUU AAA CAC 5'

PROTIEN LEU-MET-THR-THR-PRO-LEU-THR-LEU-LYS-HIS  

MUTANT TYPE

DNA 5' AAT TAC TGC TCT GGA GAG TGA ATT TGT GTT 3'

RNA 3' UUA AUG ACG ACA CCA CUC ACU UAA UCU CAA 5'

PROTEIN STOP SER GLN