Question

1. Describe the mechanism of topoisomerase type I and II. Be able to describe similarities and key differences in their mechanisms.

2. Describe in molecular detail why there is more binding informatin in the major groove vs minor groove.

Answer 1

Describe the anism a dopaisomerase type 1 and n B abe to describe similaites nd ay dgtnies in their merhanisms Ixapoisomerism Relteb_ok DNA cails is dome by Tapor somerase wo types ) Topalsomerases T Acls by making a trastnt singe aut in the back bonle o the DriA enabling the strands ta swiuel aronnd each to build up o luists. 2) Tapaiso mease ads by (making) intraduting double standed brtalss enabling one double stronded Dria Ha pass thraugh anather by tmaulng knots and enlangtmenks that an jgim uaithin enlangemenks that gtm within and botween ortA malerule Key dierntes 10poisamerases 1 ン -Generates single. Strands brake Ocurs in eukauohk Doe not atquire AP hydrolgei GH double shonds bra sampraseI

mdleaular delans why therti mt binding injatmatior inttom,0@f rooves us minararaave」 uemi 一ㄧ Large-angle -o, ma ar-Groove.conhain pllouing-stri. auing suctures A-Hydrogen. , band-arceptar- (rtitragen2 e H-band danar A Hydragen band accaphor arban graup up ybir uno roth group) nar groaue has _ TWO H bond.一acceptor one H atom