Question

3. Summarize your findings about the concentration, temperature, and pH sensitivity of lactase.

Enzyme Action An Investigation of Lactase Activity 137 PART F. EFFECT OF pH ON ENZYME ACTIVITY Activity of Lactair at Several pHs Observation with Tes Tape Glucose present Lactose pH 7 pH 2 pH 10 Glucose PART G. EFFECT OF AN INHIBITOR ON ENZYME ACTIVITY Inbibitor Efects Observation with Tes Tape Glucose present? Lactose Lactaid Ethanol Glucose 1. What is the optimum pH of the ion reaction, as shown by your data? toe the 2. Did ethanal act as an efficient inhibitor of the lactose conversion to glucose and galactose? nayba 3. Summarize your findings about the concentration, temperature, and pH sensititivity of lactase cture re 2013 C︸ lanang, Al Righh lowned May "" bHned.-araplad "pwtedto.plk..allivel.whlen 136 PART D. EFFECT OF SUBSTRATE CONCENTRATION tase at Different Lactose Concentrarions Observation with Tes Tape Glucose present? Effecthveness of Lac Untreated lactose Lactose Diluted lactose Glucose nフ PART E. EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Glucose present? Observation with Tes Tape Lactose Treated lactose Glucose Reaction at。 Observation with Tes Tape Glucose present? Lactose Treated lactose Glucose Reaction ar >75 C Observation with Tes TapeGlecose present? Lactose Treated lactose Glucose 1. How is the activity of lactase affected by the concentration of the substrate and the the reaction? dnan en 2. Could you determine an optimum temperature for the lactase reaction? 37 mene to break donn Milk sugon А+0°C, the nLe ENZYME ACTION: AN INVESTIGATION OF LACTASE ACVTIVITY AM alko- Partner RT A. NORMAL ACTIVITY OF LACTASE ame Date Results of Incubation of Lactose with Lactase Observation with Tes Tape Glucose present? Lactose + water Lactose + lactase dark ree ho Glucose 2s PART B. THE EFFECT OF SUBSTRATE ON ENZYME ACTIVITY Enzyme Activity with Different Substrates Observation with Tes Tape Glacose present? Water + lactase Lactose + actase Sucrose + lactase Glucose + water PART C. THE EFFECT OF ENZYME CONCENTRATION Activity of Enzyme at Different Dilutions Observation with Tes TapeGlucose present? Lactose Lactase Diluted Lactaid+ Glucose fenpare your results in the three different situations above. How is the activity of Iactase affected by le nature of the substrate and the concentration of the enzyme? e so streste the vl hare no llisims betwe strat也concent nen2yme 135 in en

Answer 1

LACTASE:IT IS AN ENZMYE PRODUCED BY MANY ORGANISIMS

IT IS LOCATED IN THE SMALL INTESTINE OF HUMANS AND OTHER MAMMALS

IT HELPS IN COMPLETE DIGESTION

PEOPLE WHO HAVE LESS LATASE EFFICIENCY THEY HAVE EFFECTS OF CONCENTRATION,TEMPERATURE,PH VALUE

ENZYMES ARE PROTIENN MOLECULES WHICH ACTS TO CATALYSE THE CHEMICAL REACTIONS IN LIVING THINGS.

THESE CHEMICAL REACTIONS MAKE UP THE METABOLISM OF LIVING ORGANISM

THEY ARE TWO TYPE OF MEATBOLIC REACTIONS.THEY ARE

CATABOLIC REACTION

ANABOLIC REACTION

CATABOLIC REACTION BREAK DOWN THE LARGER MOLECULES INTLO SMALLER MOLECULES

ANABOLIC REACTIONS BULID LARGER MOLECULES FROM SMALLER MOLECULES