Question

please answer all the questions

02 The rate of an enryme-catalysed reaction can be altered by the presence of an inhibitor An investigation was carried out into the effect t of an inhibitor on enzyme activity in barley root tips. Enzyme activity was measured by finding the rate at which oxygen was used by the root tips. Several groups of students carried out experiments in which the volume of oxygen used by root tips of harley seedlings was measured over a period of 2 hours Each group of students used 50 root tips from seedlings of the same age. These were placed in the same volume of a pH 6.5 buffer solution and kept at 30°C After 2 hours, the same volume of a 1% solution of an inhibitor was added to each set of root tips. The students continued to record the volume of oxygen used for a further hour. The results are shown in the graph below. Compare the enzyme activity during the first two hours of the experiment with the activity after the inhibitor was added. 3m

Answer 1

Ans-A in the first two hours the oxygen uptake was decreasing gradually up to optimal point but the inhibitor lowered the uptake of oxygen.

Ans-B non active site directed inhibition occur where inhibitor does not compete with binding of substrate but it inhibit the activity by some other action.

Ans-C the use of increaed inhibitor will inhibit the reaction more rapidly .

Ans-d the reason behind the taking of reading was for the purpose of the comparison .

Ans-e it was kept at 30 temperature because this is considered as room temperature and it is most favorable temperature for such reaction.

Ans-f buffer solution doesn't allow change in pH over a range of solution so to keep the pH constant it was used.

Ans-g the results from various student may vary because of the handling error.