Question

Plains Wanderer samples were collected from two points in time. A pre-clearing historic sample was collected from feathers stored in a museum collection. This historic sample included 40 samples from a collection of specimens shot in 1955 at a single population near Warracknabcal. Victoria. A post-clearing contemporary sample was collected in 2015 from the same area as the historic sample. This contemporary sample from 40 individuals was taken from captured and released adult birds. All samples had DNA extracted and were genotyped at three microsatellite loci At the time of sampling, a population census was made in order to estimate the size of the population remaining The census of population numbers found 84 birds. Locus 1 Locus 2 Locus 3 Allele Historic Today Historic Today Historic Today 0.000 0.620 0.138 0.075 0.312 0.250 0.150 0.225 0.000 0.000 0.000 0.000 0.150 0.174 0.350 0.250 0.360 0.251 0.288 0.170 0.150 0.000 0.040 0.010 0.075 0.049 0.000 0.000 Table 2: Population genetic diversity statistics for historic and contemporary Plains Wanderer samples Heterozygosity (He Number of alleles Toda Historic Historic 0.622 0.621 0.765 0.776 0.7340.540 0.758 4 0.594 4.00 Mean 5.33 a) Calculate the current effective population size Ne) sustained through population bottleneck 1 955-201 5 . Hint: Use the rearrangement of the equation for lost heterozygosity through a bottleneck below) 2Ne

If managers took 10 random individuals from the population today, calculate and discuss the expected number of alleles in that founder population for each locus.

Answer 1

we have the formula Ne=-1/2(Ht/Ho)^1/t -1

a) Ht=4 and Ho=5.33 so Ne=-1/2(4/5.33)^1/4-1

                                         -1/2x(0.75)0.25-1

                                          =-1/(1.5)^0.25-1

                                          =--1/0.1^0.25

b) Ht=10 and Ho=5,33 so Ne=-1/2(10/5.33)^1/10-1

                                          =-1/2x1.87 ^0.1-1

                                           =-1/2.74 ^0.1