Question

Here's a data set of bacterial growth (OD= Optical density). Please graph the growth curve as a semi-log y graph, and label appropriately. And calculate the generation time (doubling time)

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Answer 1

Answer=

The plot of growth curve i.e. optical density versus time.

1.8 16 200 250

CCalculation of generation time

Formula is

G(generation time) = (time in minutes or hours)/ n(number of generations)

G = t/n

t = time interval in hours or minutes.

B= number of bacteria at the beginning of a time interval

b=number of bacteria at the end  of a time interval

n = number of generations

b = B X 2ⁿ

n = log b - logB /0.301

n = 3.3 log b/B

G = t / 3.3 log b/B