Question

The Hill plot is shown below for both Hemoglobin (Hb) and Myoglobin (Mb). What is the value of Hill coefficient for each protein and how do you interpret each Hill curve? Please work out how to find n for Hemoglobin.

2. The Hill plot is shown below for both Hemoglobin (Hb) and Myoglobin (Mb). What is the value of Hill coefficient for each protein and how do you interpret each Hill curve? [15] n 42, 43801 Mb X -2 -1 2 3 0 1 logpo2

Answer 1

Physiology of beood og affenity, co-operatively and Bohn's effect are the factors that affect binding capacity of Hb and Mb with oa. Relative og binding properties of me e tb és to measure fraction of oxygenated vien site as function of partial pressure of a g. og at one subunit influences 2 benaling at others that in binding is co-operative Hell's equations ar e To quantify the affinity of heem peatein Joe on it to plot the log of ratio of G o concentration of oxigenated to decuygenated sites. rub to m boe - 1 кг (ron] saturation o= mb ] [ub total. [ris a total = {my] + [m3 (2] - (mg + k, [p] [2] [mb] total = {m} [1+ k, [2]

a = [meo2] (my] [1+k, low]] kilomy] [2] It bio] Causs on ki027 Itk, [2] 1-o the forms. 1- O m=3 for Haeme globen. value of a depends on pH at playriological pre meas. Me log Po,
hills coeffiecent for hb is 3

Thus gives the sigmoidal curve for hb that means binding of oxygen at one subunit affects the oxygen binding at other units which shows the co operative effect

Where as graph of mb is hyperbolic which shows the simple equation that one O2 binds to 1Fe