Question

please help me with this! im so lost and confused!

Name Lab Report Purpose: Describe the purpose of the lab of bacteria. a. Also, to show the efectivaness oFvarious onthbts against Grom postie ard Gram-nie pacteri a Exercise 1: Disinfectants Organism 1: Growth Scale: 0- No Growth; 5-Maximum Growth Be sure to record the chemical or product you tested and complete this table by obtaining data from Times of ea ram Exposure Name of the Name of the Name of the cleaner: Name of the Name of the cleaner: cleaner: cleaner: 10% bleach cleaner: 0 min 1 min 5 min 10 min other groups in your class Organism 2: Growth Scale: 0 No Growth; 5-Maximum Growth Be sure to record the chemical or product you tested and complete this table by obtaining data from other groups in your class Times of Organism Used: E. coli (Gram negative) Exposure Name of the Name of the Name of the Name of the Name of the | cleaner: o cleaner: 10% bleach cleaner: cleaner: cleaner: 0 min 1 min 5 min 10 min Questions: 1. Was your test chemical effective? Briefly explair. 2. Was your test chemical more effective that the lab disinfectant? Briefly explain. 3. Was your test chemical bactericidal or bacteriostatic? Look up the label of the preparation you tested. What is (are) the active ingredient(s)? 4. Using your textbook or another reference, find the method of action of the active ingredient(s) in the test substance 5. BIOS242, Week 4, Lab 1 Name: Exercise 2: Antimicrobial Agents B. subtilis E. colS, marcescens s. epidermidis Antimicrobial Disk S, I or Zone Size S,l, or R Zone Size S, I, or R R (mm) Agent Code Zon e S S,, or Zone Size Size R Penicilin PIO Ampicylio AM Questions Which antimicrobial agents were most effective against each organism? 1. to nn antimicrobial agent? Why?

Answer 1

5.

Penicillin:

This product is part of the b-Lactam antibiotics which means that It’s able to inhibit the formation of peptidoglycan cross links in the bacterial cell wall leading to a break down at the bacteria cell wall and decreasing their grow. This is accomplish though the binding of b-lactam ring of penicillin to the enzyme DD-transpeptidase affecting the catalytic effect of this enzyme.

Tetracycline:

This product is able to inhibit protein synthesis by blocking the attachment of aminoacyl-tRNA to the ribosome. Tetracycline could bind to the 30S subunit of microbial ribosomes, affecting the processing of tRNA.

Ampicillin:

This product belong to the same group of penicillin because of its beta-lactam portion. It’s able to penetrate gram positive and several gram negative bacteria. The main difference between Ampicillin and Penicillin is that ampicillin has an additional amino group on its structure which helps to pass trough the pores of the outer membrane of gram negative bacteria.