Question

a. What organisms were the tubulin and the cyclin primary antibodies raised in?

b. What was the purpose of the tubulin control? What other proteins might you have used instead of tubulin?

The aim of this week’s experiment is to verify how M-cyclin is varying within the synchronised tobacco BY2 cell culture. Protein levels will be assays using a dot blot immunoassay in which a crude protein extract is coated onto a nitrocellulose membrane and probed with specific antibodies. This is a simplified version of the western blot assay, common in cell biology, in which proteins are first separated by molecular weight on a gel, transferred to nitrocellulose membrane and then probed with antibodies.

Answer 1

Answer.a) Tobacco is used for raising the tubulin and cycling primary antibody as these experiment have Tobacco BY2 cells.

B) Tubulin is used as control because it is housekeeping protein that synthesis regularly. Also we can use actin as control