Question

For the four reasons listed below, please explain a solution for each reason.

Thank you in advance!

QUESTION 8 In the production of iPSCs, proteins that regulate transcription are injected into differentiated cells to revert them back to stem cells However, the efficiency of de-differentiation ie: returning to a stem cell like state is typically less than 1%. Give 4 reasons why in ecting the transcription factor proteins into the cell may not be enough to lead to change the cell state. In other words, why is the presence of the protein alone insufficient? Then, choose one of your 4 reasons and give a solution that would solve that problem. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). 1. Histones prevent transcription factors from binding cis-factors. 2. Repressors are present that prevent transcription even in the presence of transcription factors. 3. RNA is transcribed, but not spliced properly 4. RNA is transcribed and protein is translated, but the protein is not properly localized or post-translationally modified Path: p» span Words:48

Answer 1

2, Repressors are present that prevent transcription even in the presence of transcription factors..

MicroRNA molecules can block expression of Repressors of four transcription factors, when additional reprogramming induced even in the absence of added exogenous transcription factors..ipscs form teratoma..this teratoma formation is considered a major abstract to stem cells..