What is next generation sequencing? What are advantages and disadvantages? How does it work?
Next generation sequencing:
Advantage:
Disadvantage:
Next generation sequencing works:
What is next generation sequencing? What are advantages and disadvantages? How does it work?
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11. What is next-generation sequencing? 도 있다.
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? The complete DNA strands are synthesized then sequenced. Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated. The sequencing occurs during S phase of the cell cycle. RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined. Incorporated nucleotides are determined while they are being added to a growing DNA strand.
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list the methods used in next generation sequencing
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...