list the methods used in next generation sequencing
list the methods used in next generation sequencing
Homework Answers
The methods used in next generation sequencing(NGS) are:
- Illumina(Solexa) sequencing
- Roche 454 sequencing
- Ion Torrent: Proton/PGM sequencing
In illumina sequencing, it works by simultaneously identifying DNA bases. This is possible as each base emits a unique florescent signal. Now these are added to a nucleic acid chain and sequenced.
In Roche 454 sequencing, which is based on pyrosequencing which is a technique which dtetects pyrophosphate release. Here we are using the florescence, after nucleotides are incorporated by polymerase to a new strand of DNA.
Ion Torrent sequencing measures the H+ ions which are released directly by the incorporation of individual bases by DNA polymerase. It is different from the previous two methods because it does not measure light.
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? The complete DNA strands are synthesized then sequenced. Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated. The sequencing occurs during S phase of the cell cycle. RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined. Incorporated nucleotides are determined while they are being added to a growing DNA strand.
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
11. What is next-generation sequencing? 도 있다.
11. What is next-generation sequencing? 도 있다.
Compare and contract next generation sequencing. 1.Pyrosequencing 2.454 Technology 3.Illumina sequencing plz give details
Compare and contract next generation sequencing. 1.Pyrosequencing 2.454 Technology 3.Illumina sequencing plz give details
What is next generation sequencing? What are advantages and disadvantages? How does it work?
What is next generation sequencing? What are advantages and disadvantages? How does it work?
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What is the name of method to screen for gene expression profiles using next generation sequencing technique? All expressed genes can be analyzed by this method.
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next...
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
(I only need the answer for #2) High-throughput (next-generation) sequencing techniques are making ‘community genet- ics’...
(I only need the answer for #2) High-throughput (next-generation) sequencing techniques are making ‘community genet- ics’ programmes more widely applicable, because cheap tests can be used to screen population members for asymptomatic heterozygote carriers of many different disease alleles. 1- What are the potential benefits and costs of this community genetics approach? 2- Give at least 3 benefits ( pros) and 3 costs (cons) ?
You are planning an NGS(Next Generation Sequencing) experiment to study cancer samples that could occur due...
You are planning an NGS(Next Generation Sequencing) experiment to study cancer samples that could occur due to over-expression or translocation leading to a fusion gene. Which NGS method is appropriate for this study? Explain your answer with examples in support of your answer: A. WGS B. ChIPSeq C. WES D. RNASeq
1. Compare and contrast first generation sequencing techniques (Sanger sequencing and pyrosequencing) and the second generation...
1. Compare and contrast first generation sequencing techniques (Sanger sequencing and pyrosequencing) and the second generation sequencing technique ForenSeq using the MiSeq. Include a discussion of reagents, time, cost, optimal sequence length, detection technologies, data output and post-testing analysis in your discussion. Tabulate your answers and be sure to complete the table. 2. Describe the processes that are occurring in PCR1 and PCR2. Why are each of these steps important. Describe the roles of i5 and i7, forward and reverse...
11. What is next-generation sequencing? 도 있다.
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
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