Question

Research Paper Written Assignment # 2 Title: Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model Authors: Nir Pillarl, Avital Luba Polskyl and Noam Shomron 1. What is a micro RNA? 2. What is the function of mir96? 3. What are the oncogenes in this paper? Define oncogene 4. What happens to LCP1 and ABCE1 when mir96 is OE in a migration and invasion assay using of 4TI breast cancer cells S. Explain LCPl gene expression in human breast cancer tissues 6. What would you expect to be the expression patterns of ABCE1 in breast cancer tissue versus nomal breast tissue? 7. What is meant when the paper says that mir96 has dual inhibition? Briefly explain 8. What does the term "down regulation" mean?

Answer 1

Answer 1)

1. The micro-RNA is a small stretch of nucleotide that plays a vital role in the regulation during post-translational event.
2. micro-RNA is a non-coding which indicates that it does not encode for any product and hence can act as marker.

Answer 2)

1. mir96 is the micro-RNA 96 which has been mentioned in the abstract. It is a short stretch of RNA and is involved in regulation by means of an inhibitory effect.
2. Breast cancer is associated with uncontrolled division of cells and this process is suppressed by the mir96 sequence. Thus, it has a regulatory role.

Answer 3)

1. ABCE-1 and LCP-1 are the oncogenes which are involved in cancer progression and metastasis.
2. An oncogene can be defined as a gene which if activated would lead to uncontrolled cell division resulting in cancer.